The carboxyl-terminal two-thirds of the ADP/ATP carrier polypeptide contains sufficient information to direct translocation into mitochondria - PubMed

Affiliations

• PMID: 2822702

Free article

The carboxyl-terminal two-thirds of the ADP/ATP carrier polypeptide contains sufficient information to direct translocation into mitochondria

N Pfanner et al. J Biol Chem. 1987.

Free article

Abstract

The precursor of the mitochondrial inner membrane protein ADP/ATP carrier is cytoplasmically synthesized without an amino-terminal peptide extension. We constructed a truncated precursor lacking the 103 amino acids from the amino terminus (about a third of the protein). Import of the truncated precursor into mitochondria showed the import characteristics of the authentic precursor, including nucleoside triphosphate dependence, requirement for a protease-sensitive component on the mitochondrial surface, two-step specific binding to the outer membrane, and membrane potential-dependent translocation into the inner membrane. We conclude that, in contrast to all other mitochondrial precursor proteins studied so far, domains of the ADP/ATP carrier distant from the amino terminus can carry specific targeting information for transport into mitochondria.

Similar articles

• Distinct steps in the import of ADP/ATP carrier into mitochondria.

  Pfanner N, Neupert W. Pfanner N, et al. J Biol Chem. 1987 Jun 5;262(16):7528-36. J Biol Chem. 1987. PMID: 3034898

• Transport of proteins into mitochondria. Posttranslational transfer of ADP/ATP carrier into mitochondria in vitro.

  Zimmermann R, Neupert W. Zimmermann R, et al. Eur J Biochem. 1980 Aug;109(1):217-29. doi: 10.1111/j.1432-1033.1980.tb04787.x. Eur J Biochem. 1980. PMID: 6250835

• Transfer of proteins into mitochondria. Precursor to the ADP/ATP carrier binds to receptor sites on isolated mitochondria.

  Zwizinski C, Schleyer M, Neupert W. Zwizinski C, et al. J Biol Chem. 1983 Apr 10;258(7):4071-4. J Biol Chem. 1983. PMID: 6300074

• The ADP and ATP transport in mitochondria and its carrier.

  Klingenberg M. Klingenberg M. Biochim Biophys Acta. 2008 Oct;1778(10):1978-2021. doi: 10.1016/j.bbamem.2008.04.011. Epub 2008 May 2. Biochim Biophys Acta. 2008. PMID: 18510943 Review.

• The role and structure of mitochondrial carriers.

  Kunji ER. Kunji ER. FEBS Lett. 2004 Apr 30;564(3):239-44. doi: 10.1016/S0014-5793(04)00242-X. FEBS Lett. 2004. PMID: 15111103 Review.

Cited by

• The role of the Tim8p-Tim13p complex in a conserved import pathway for mitochondrial polytopic inner membrane proteins.

  Curran SP, Leuenberger D, Schmidt E, Koehler CM. Curran SP, et al. J Cell Biol. 2002 Sep 16;158(6):1017-27. doi: 10.1083/jcb.200205124. Epub 2002 Sep 9. J Cell Biol. 2002. PMID: 12221072 Free PMC article.

• Role of Mitochondrial Protein Import in Age-Related Neurodegenerative and Cardiovascular Diseases.

  Bogorodskiy A, Okhrimenko I, Burkatovskii D, Jakobs P, Maslov I, Gordeliy V, Dencher NA, Gensch T, Voos W, Altschmied J, Haendeler J, Borshchevskiy V. Bogorodskiy A, et al. Cells. 2021 Dec 14;10(12):3528. doi: 10.3390/cells10123528. Cells. 2021. PMID: 34944035 Free PMC article. Review.

• Mitochondrial uptake of thiamin pyrophosphate: physiological and cell biological aspects.

  Subramanian VS, Nabokina SM, Lin-Moshier Y, Marchant JS, Said HM. Subramanian VS, et al. PLoS One. 2013 Aug 30;8(8):e73503. doi: 10.1371/journal.pone.0073503. eCollection 2013. PLoS One. 2013. PMID: 24023687 Free PMC article.

• An ABC transporter in the mitochondrial inner membrane is required for normal growth of yeast.

  Leighton J, Schatz G. Leighton J, et al. EMBO J. 1995 Jan 3;14(1):188-95. doi: 10.1002/j.1460-2075.1995.tb06989.x. EMBO J. 1995. PMID: 7828591 Free PMC article.

• Mitochondrial targeting of yeast apoiso-1-cytochrome c is mediated through functionally independent structural domains.

  Nye SH, Scarpulla RC. Nye SH, et al. Mol Cell Biol. 1990 Nov;10(11):5763-71. doi: 10.1128/mcb.10.11.5763-5771.1990. Mol Cell Biol. 1990. PMID: 2172785 Free PMC article.

Publication types

MeSH terms

Substances