Involvement of GTP-binding "G" proteins in transport through the Golgi stack - PubMed

Involvement of GTP-binding "G" proteins in transport through the Golgi stack

P Melançon et al. Cell. 1987.

Free article

Abstract

GTP gamma S irreversibly inhibits protein transport between successive compartments of the Golgi stack in a cell-free system. Fluoride, potentiated by the addition of aluminum ion, also causes a strong inhibition. These are hallmarks of the involvement of a guanine nucleotide-binding or regulatory "G" protein. Inhibition by GTP gamma S requires a cytosolic inhibitory factor that binds to Golgi membranes during inhibition. Preincubation experiments reveal that GTP gamma S blocks the function of acceptor Golgi but not donor Golgi membranes. More specifically, a processing step in between vesicle attachment and the actual fusion event seems to be affected. Electron microscopy demonstrates a corresponding 5-fold accumulation of non-clathrin-coated buds and vesicles associated with the Golgi cisternae during inhibition by GTP gamma S.

Similar articles

• Calcium and GTP: essential components in vesicular trafficking between the endoplasmic reticulum and Golgi apparatus.

  Beckers CJ, Balch WE. Beckers CJ, et al. J Cell Biol. 1989 Apr;108(4):1245-56. doi: 10.1083/jcb.108.4.1245. J Cell Biol. 1989. PMID: 2538479 Free PMC article.

• Cell-free transport to distinct Golgi cisternae is compartment specific and ARF independent.

  Happe S, Weidman P. Happe S, et al. J Cell Biol. 1998 Feb 9;140(3):511-23. doi: 10.1083/jcb.140.3.511. J Cell Biol. 1998. PMID: 9456313 Free PMC article.

• Coatomer vesicles are not required for inhibition of Golgi transport by G-protein activators.

  Happe S, Cairns M, Roth R, Heuser J, Weidman P. Happe S, et al. Traffic. 2000 Apr;1(4):342-53. doi: 10.1034/j.1600-0854.2000.010407.x. Traffic. 2000. PMID: 11208119

• Analysis of protein transport through the Golgi in a reconstituted cell-free system.

  Wattenberg BW. Wattenberg BW. J Electron Microsc Tech. 1991 Feb;17(2):150-64. doi: 10.1002/jemt.1060170204. J Electron Microsc Tech. 1991. PMID: 1901603 Review.

• The protein machinery of vesicle budding and fusion.

  Rothman JE. Rothman JE. Protein Sci. 1996 Feb;5(2):185-94. doi: 10.1002/pro.5560050201. Protein Sci. 1996. PMID: 8745395 Free PMC article. Review.

Cited by

• The many routes of Golgi-dependent trafficking.

  Boncompain G, Perez F. Boncompain G, et al. Histochem Cell Biol. 2013 Sep;140(3):251-60. doi: 10.1007/s00418-013-1124-7. Epub 2013 Jul 12. Histochem Cell Biol. 2013. PMID: 23846822 Review.

• ADP-ribosylation factor, a small GTP-binding protein, is required for binding of the coatomer protein beta-COP to Golgi membranes.

  Donaldson JG, Cassel D, Kahn RA, Klausner RD. Donaldson JG, et al. Proc Natl Acad Sci U S A. 1992 Jul 15;89(14):6408-12. doi: 10.1073/pnas.89.14.6408. Proc Natl Acad Sci U S A. 1992. PMID: 1631136 Free PMC article.

• Purification of an N-ethylmaleimide-sensitive protein catalyzing vesicular transport.

  Block MR, Glick BS, Wilcox CA, Wieland FT, Rothman JE. Block MR, et al. Proc Natl Acad Sci U S A. 1988 Nov;85(21):7852-6. doi: 10.1073/pnas.85.21.7852. Proc Natl Acad Sci U S A. 1988. PMID: 3186695 Free PMC article.

• Detection of GTP-binding proteins in purified derivatives of rough endoplasmic reticulum.

  Lanoix J, Roy L, Paiement J. Lanoix J, et al. Biochem J. 1989 Sep 1;262(2):497-503. doi: 10.1042/bj2620497. Biochem J. 1989. PMID: 2508629 Free PMC article.

Publication types

MeSH terms

Substances