Molecular cloning of the cDNA for the large subunit of the high-Ca2+-requiring form of human Ca2+-activated neutral protease - PubMed

Affiliations

• PMID: 2852952
• DOI: 10.1021/bi00421a022

Comparative Study

Molecular cloning of the cDNA for the large subunit of the high-Ca2+-requiring form of human Ca2+-activated neutral protease

S Imajoh et al. Biochemistry. 1988.

Abstract

A nearly full-length cDNA clone for the large subunit of high-Ca2+-requiring Ca2+-activated neutral protease (mCANP) from human tissues has been isolated. The deduced protein, determined for the first time as an mCANP, has essentially the same structural features as those revealed previously for the large subunits of the low-Ca2+-requiring form (muCANP) [Aoki, K., Imajoh, S., Ohno, S., Emori, Y., Koike, M., Kosaki, G., & Suzuki, K. (1986) FEBS Lett. 205, 313-317] and chicken CANP [Ohno, S., Emori, Y., Imajoh, S., Kawasaki, H., Kisaragi, M., & Suzuki, K. (1984) Nature (London) 312, 566-570]. Namely, the protein, comprising 700 amino acid residues, is characterized by four domains, containing a cysteine protease like domain and a Ca2+-binding domain. The overall amino acid sequence similarities of the mCANP large subunit with those of human muCANP and chicken CANP are 62% and 66%, respectively. These values are slightly lower than that observed between muCANP and chicken CANP (70%). Local sequence similarities vary with the domain, 73-78% in the cysteine protease like domain and 48-65% in the Ca2+-binding domain. These results suggest that CANPs with different Ca2+ sensitivities share a common evolutionary origin and that their regulatory mechanisms are similar except for the Ca2+ concentrations required for activation.

Similar articles

• Isolation and sequence analyses of cDNA clones for the large subunits of two isozymes of rabbit calcium-dependent protease.

  Emori Y, Kawasaki H, Sugihara H, Imajoh S, Kawashima S, Suzuki K. Emori Y, et al. J Biol Chem. 1986 Jul 15;261(20):9465-71. J Biol Chem. 1986. PMID: 2424911

• Complete amino acid sequence of the large subunit of the low-Ca2+-requiring form of human Ca2+-activated neutral protease (muCANP) deduced from its cDNA sequence.

  Aoki K, Imajoh S, Ohno S, Emori Y, Koike M, Kosaki G, Suzuki K. Aoki K, et al. FEBS Lett. 1986 Sep 15;205(2):313-7. doi: 10.1016/0014-5793(86)80919-x. FEBS Lett. 1986. PMID: 3017764

• Molecular cloning of a novel mammalian calcium-dependent protease distinct from both m- and mu-types. Specific expression of the mRNA in skeletal muscle.

  Sorimachi H, Imajoh-Ohmi S, Emori Y, Kawasaki H, Ohno S, Minami Y, Suzuki K. Sorimachi H, et al. J Biol Chem. 1989 Nov 25;264(33):20106-11. J Biol Chem. 1989. PMID: 2555341

• Autolysis of calcium-activated neutral protease of chicken skeletal muscle.

  Suzuki K, Tsuji S, Ishiura S, Kimura Y, Kubota S, Imahori K. Suzuki K, et al. J Biochem. 1981 Dec;90(6):1787-93. doi: 10.1093/oxfordjournals.jbchem.a133656. J Biochem. 1981. PMID: 6277879

• Calpain chronicle--an enzyme family under multidisciplinary characterization.

  Sorimachi H, Hata S, Ono Y. Sorimachi H, et al. Proc Jpn Acad Ser B Phys Biol Sci. 2011;87(6):287-327. doi: 10.2183/pjab.87.287. Proc Jpn Acad Ser B Phys Biol Sci. 2011. PMID: 21670566 Free PMC article. Review.

Cited by

• IP-10 inhibits epidermal growth factor-induced motility by decreasing epidermal growth factor receptor-mediated calpain activity.

  Shiraha H, Glading A, Gupta K, Wells A. Shiraha H, et al. J Cell Biol. 1999 Jul 12;146(1):243-54. doi: 10.1083/jcb.146.1.243. J Cell Biol. 1999. PMID: 10402474 Free PMC article.

• Neutrophil signaling during myocardial infarction wound repair.

  Daseke MJ 2nd, Chalise U, Becirovic-Agic M, Salomon JD, Cook LM, Case AJ, Lindsey ML. Daseke MJ 2nd, et al. Cell Signal. 2021 Jan;77:109816. doi: 10.1016/j.cellsig.2020.109816. Epub 2020 Oct 24. Cell Signal. 2021. PMID: 33122000 Free PMC article. Review.

• Increased mRNA levels for components of the lysosomal, Ca2+-activated, and ATP-ubiquitin-dependent proteolytic pathways in skeletal muscle from head trauma patients.

  Mansoor O, Beaufrere B, Boirie Y, Ralliere C, Taillandier D, Aurousseau E, Schoeffler P, Arnal M, Attaix D. Mansoor O, et al. Proc Natl Acad Sci U S A. 1996 Apr 2;93(7):2714-8. doi: 10.1073/pnas.93.7.2714. Proc Natl Acad Sci U S A. 1996. PMID: 8610106 Free PMC article.

• Chromosomal localization of the porcine skeletal muscle calpain gene.

  Briley GP, Riggs PK, Womack JE, Hancock DL, Bidwell CA. Briley GP, et al. Mamm Genome. 1996 Mar;7(3):226-8. doi: 10.1007/s003359900062. Mamm Genome. 1996. PMID: 8833248 No abstract available.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc
  • GlyGen glycoinformatics resource

• Miscellaneous

  • NCI CPTAC Assay Portal