Characterization of cDNA coding for human factor XIIIa - PubMed

Characterization of cDNA coding for human factor XIIIa

U Grundmann et al. Proc Natl Acad Sci U S A. 1986 Nov.

Abstract

A cDNA library prepared from human placenta has been screened for sequences coding for factor XIIIa, the enzymatically active subunit of the factor XIII complex that stabilizes blood clots through crosslinking of fibrin molecules. Two oligonucleotides, based on the amino acid sequences of tryptic peptides of factor XIIIa, were used as hybridization probes. Of 0.36 X 10(6) independent recombinants, 1 clone was identified that hybridized to both probes. The insert of 1704 base pairs coded for the amino-terminal 541 amino acid residues of the mature factor XIIIa molecule. Blot-hybridization analysis using this cDNA as a probe showed that the factor XIIIa mRNA from placenta has a size of approximately 4000 bases. The insert was used to rescreen cDNA libraries and to identify further factor XIIIa-specific sequences. The total length of the isolated factor XIIIa cDNA is 3905 bases, and it codes for a protein of 732 amino acids. In spite of the presence of factor XIII in blood plasma, we could not identify a leader sequence typical for secreted proteins.

Similar articles

• Primary structure of blood coagulation factor XIIIa (fibrinoligase, transglutaminase) from human placenta.

  Takahashi N, Takahashi Y, Putnam FW. Takahashi N, et al. Proc Natl Acad Sci U S A. 1986 Nov;83(21):8019-23. doi: 10.1073/pnas.83.21.8019. Proc Natl Acad Sci U S A. 1986. PMID: 2877456 Free PMC article.

• Amino acid sequence of the a subunit of human factor XIII.

  Ichinose A, Hendrickson LE, Fujikawa K, Davie EW. Ichinose A, et al. Biochemistry. 1986 Nov 4;25(22):6900-6. doi: 10.1021/bi00370a025. Biochemistry. 1986. PMID: 3026437

• Amino acid sequence of the b subunit of human factor XIII, a protein composed of ten repetitive segments.

  Ichinose A, McMullen BA, Fujikawa K, Davie EW. Ichinose A, et al. Biochemistry. 1986 Aug 12;25(16):4633-8. doi: 10.1021/bi00364a027. Biochemistry. 1986. PMID: 3021194

• Characterization of the gene for the a subunit of human factor XIII (plasma transglutaminase), a blood coagulation factor.

  Ichinose A, Davie EW. Ichinose A, et al. Proc Natl Acad Sci U S A. 1988 Aug;85(16):5829-33. doi: 10.1073/pnas.85.16.5829. Proc Natl Acad Sci U S A. 1988. PMID: 2901091 Free PMC article.

• Cloning and sequence analysis of cDNA clones for bovine aortic-endothelial-cell transglutaminase.

  Nakanishi K, Nara K, Hagiwara H, Aoyama Y, Ueno H, Hirose S. Nakanishi K, et al. Eur J Biochem. 1991 Nov 15;202(1):15-21. doi: 10.1111/j.1432-1033.1991.tb16338.x. Eur J Biochem. 1991. PMID: 1682150

Cited by

• Molecular basis for subtypic differences of the "a" subunit of coagulation factor XIII with description of the genesis of the subtypes.

  Suzuki K, Iwata M, Ito S, Matsui K, Uchida A, Mizoi Y. Suzuki K, et al. Hum Genet. 1994 Aug;94(2):129-35. doi: 10.1007/BF00202857. Hum Genet. 1994. PMID: 7913909

• Revisiting the mechanism of coagulation factor XIII activation and regulation from a structure/functional perspective.

  Gupta S, Biswas A, Akhter MS, Krettler C, Reinhart C, Dodt J, Reuter A, Philippou H, Ivaskevicius V, Oldenburg J. Gupta S, et al. Sci Rep. 2016 Jul 25;6:30105. doi: 10.1038/srep30105. Sci Rep. 2016. PMID: 27453290 Free PMC article.

• Isolation of a polymorphic genomic clone from chromosome 7. Physical and genetic linkage studies to markers around the cystic fibrosis locus.

  Davies KA, Lorand L, Waterfield M, Wainwright B, Farrall M, Williamson R. Davies KA, et al. Hum Genet. 1987 Oct;77(2):122-6. doi: 10.1007/BF00272377. Hum Genet. 1987. PMID: 2888718

• Complete cDNA sequence encoding human beta-galactoside alpha-2,6-sialyltransferase.

  Grundmann U, Nerlich C, Rein T, Zettlmeissl G. Grundmann U, et al. Nucleic Acids Res. 1990 Feb 11;18(3):667. doi: 10.1093/nar/18.3.667. Nucleic Acids Res. 1990. PMID: 2408023 Free PMC article. No abstract available.

• Extensive DNA polymorphism at the factor XIIIa (F13A) locus and linkage to HLA.

  Zoghbi HY, Daiger SP, McCall A, O'Brien WE, Beaudet AL. Zoghbi HY, et al. Am J Hum Genet. 1988 Jun;42(6):877-83. Am J Hum Genet. 1988. PMID: 2897163 Free PMC article.

References

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc
  • GlyGen glycoinformatics resource