The regulatory role of IL-6R in hepatitis B-associated fibrosis and cirrhosis - PubMed
- ️Sun Jan 01 2017
The regulatory role of IL-6R in hepatitis B-associated fibrosis and cirrhosis
Y Chen et al. Braz J Med Biol Res. 2017.
Abstract
This study investigated the expression and regulation of IL-6R in hepatitis B-associated moderate hepatic fibrosis and cirrhosis. Liver tissues, peripheral blood monocytes (PBMs) and serum were collected from 26 hepatitis B patients with liver fibrosis and 35 hepatitis B patients with liver cirrhosis. The levels of Il-6r mRNA expression in these samples were examined by quantitative real-time PCR and IL-6R protein levels were analyzed by western blot and ELISA. MiRNAs that regulate IL-6R expression were predicted by bioinformatics analysis, and validated by dual luciferase reporter assay. Compared with the hepatic fibrosis group, IL-6R was significantly upregulated at both mRNA and protein levels in liver tissues, PBMs and serum samples from the hepatic cirrhosis group (P<0.05). The 3'UTR of Il-6r mRNA was predicted to contain a miR-30b binding site and IL-6R was identified as a possible target of miR-30b. MiR-30b expression was significantly downregulated in samples from hepatic cirrhosis patients compared with hepatic fibrosis patients (P<0.05). In conclusion, IL-6R was upregulated while miR-30b was decreased in patients with liver cirrhosis. The miR-30 can directly regulate the expression of IL-6R.
Figures





Similar articles
-
Zhong X, Chen Y, Yao C, Xu L, Peng Y, Yang Q, Zhao M, Guo X. Zhong X, et al. Nucleosides Nucleotides Nucleic Acids. 2020;39(8):1162-1178. doi: 10.1080/15257770.2020.1780439. Epub 2020 Jul 9. Nucleosides Nucleotides Nucleic Acids. 2020. PMID: 32643523
-
Zhang N, Zhang Q, Yang W, Miao L, Wang N, Wei S, Ge J, Li X, Wu J. Zhang N, et al. Exp Ther Med. 2019 Apr;17(4):3233-3238. doi: 10.3892/etm.2019.7280. Epub 2019 Feb 15. Exp Ther Med. 2019. PMID: 30936998 Free PMC article.
-
Yang Z, Peng Y, Yang S. Yang Z, et al. Exp Ther Med. 2019 Jun;17(6):4670-4676. doi: 10.3892/etm.2019.7490. Epub 2019 Apr 16. Exp Ther Med. 2019. PMID: 31086599 Free PMC article.
-
Soresi M, Giannitrapani L, D'Antona F, Florena AM, La Spada E, Terranova A, Cervello M, D'Alessandro N, Montalto G. Soresi M, et al. World J Gastroenterol. 2006 Apr 28;12(16):2563-8. doi: 10.3748/wjg.v12.i16.2563. World J Gastroenterol. 2006. PMID: 16688802 Free PMC article.
-
Shen XB, Zhang SH, Li HY, Chi XD, Jiang L, Huang QL, Xu SH. Shen XB, et al. Med Sci Monit. 2018 Sep 11;24:6349-6358. doi: 10.12659/MSM.908555. Med Sci Monit. 2018. PMID: 30203815 Free PMC article.
Cited by
-
Huang SP, Chen S, Ma YZ, Zhou A, Jiang H, Wu P. Huang SP, et al. Evid Based Complement Alternat Med. 2022 Jun 6;2022:9363131. doi: 10.1155/2022/9363131. eCollection 2022. Evid Based Complement Alternat Med. 2022. PMID: 35707473 Free PMC article.
-
let-7a suppresses liver fibrosis via TGFβ/SMAD signaling transduction pathway.
Zhang Y, Guo J, Li Y, Jiao K, Zhang Y. Zhang Y, et al. Exp Ther Med. 2019 May;17(5):3935-3942. doi: 10.3892/etm.2019.7457. Epub 2019 Apr 1. Exp Ther Med. 2019. PMID: 31007736 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials