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Effect of radiotherapy on activity and concentration of serum paraoxonase-1 in breast cancer patients - PubMed

  • ️Sun Jan 01 2017

. 2017 Nov 27;12(11):e0188633.

doi: 10.1371/journal.pone.0188633. eCollection 2017.

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Effect of radiotherapy on activity and concentration of serum paraoxonase-1 in breast cancer patients

Meritxell Arenas et al. PLoS One. 2017.

Abstract

Paraoxonase-1 (PON1) is an intra-cellular antioxidant enzyme found also in the circulation associated with high-density lipoproteins. The activity of this enzyme has been shown to be decreased in breast cancer (BC) patients. The aims of our study were to investigate the changes produced by radiotherapy (RT) on activity and concentration of serum PON1 in BC patients, and to evaluate the observed variations in relation to clinical and pathological characteristics of patients and tumors, and the response to treatment. We studied 200 women with BC who were scheduled to receive RT following excision of the tumor. Blood for analyses was obtained before and after the irradiation procedure. The control group was composed of 200 healthy women. Relative to control, BC patients had significantly lower serum PON1 activities pre-RT, while PON1 concentrations were at similar levels. RT was associated with a significant increase in serum PON1 activities and concentrations. We observed significant differences in serum PON1 concentrations post-RT between patients with luminal A or luminal B tumors. Serum PON1 concentration post-RT was markedly lower in BC patients with metastases. We conclude that benefit from RT accrues to the BC patients not only through its direct effect on cancer cells but also indirectly by improving the organism's anti-oxidant defense mechanisms. In addition, our preliminary evidence suggests that the measurement of serum PON1 concentration post-RT could be an efficient prognostic biomarker, and may be used as an index of the efficacy of the RT.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Serum paraoxonase (PON1) activities and concentrations pre- and post-radiotherapy (RT) in patients with breast cancer (BC) segregated with respect to the investigated PON1 gene polymorphisms.

*: P < 0.05 with respect to the wild-type isoform (QQ and LL, respectively).

Fig 2
Fig 2. Serum PON1 concentrations post-radiotherapy (RT) in patients with breast cancer with or without metastasis (left panel). Receiver operating characteristics (ROC) plots of serum PON1 concentrations post-RT in breast cancer patients according to whether they had metastasis or not (right panel).

AUC: Area under the curve.

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References

    1. Simone V, D'Avenia M, Argentiero A, Felici C, Rizzo FM, De Pergola G, et al. Silvestris F. Obesity and Breast Cancer: Molecular interconnections and potential clinical applications. Oncologist. 2016; 21: 404–417. doi: 10.1634/theoncologist.2015-0351 - DOI - PMC - PubMed
    1. Kaya MO, Sinan S, Güler ÖÖ, Arslan O. Is there a relation between genetic susceptibility with cancer? A study about paraoxanase (PON1) enzyme activity in breast cancer cases. J Enzyme Inhib Med Chem. 2016; 31: 1349–1355. doi: 10.3109/14756366.2015.1134523 - DOI - PubMed
    1. Elkiran ET, Mar N, Aygen B, Gursu F, Karaoglu A, Koca S. Serum paraoxonase and arylesterase activities in patients with lung cancer in a Turkish population. BMC Cancer. 2007; 7: 48 doi: 10.1186/1471-2407-7-48 - DOI - PMC - PubMed
    1. Barrera G. Oxidative stress and lipid peroxidation products in cancer progression and therapy. ISRN Oncol. 2012; 2012: 137289 doi: 10.5402/2012/137289 - DOI - PMC - PubMed
    1. Chander R, Kapoor NK. High-density lipoprotein is a scavenger of superoxide anions. Biochem Pharmacol. 1990; 40: 1663–1665. - PubMed

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Grants and funding

This work was funded by a grant from Associació Oncològica Dr. Amadeu Pelegrí, Salou, Spain, AODAP/2015. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.