pubmed.ncbi.nlm.nih.gov

Genetic complementation in the Xenopus oocyte: co-expression of sea urchin histone and U7 RNAs restores 3' processing of H3 pre-mRNA in the oocyte - PubMed

Genetic complementation in the Xenopus oocyte: co-expression of sea urchin histone and U7 RNAs restores 3' processing of H3 pre-mRNA in the oocyte

K Strub et al. EMBO J. 1986 Jul.

Abstract

Efficient 3' processing of sea urchin histone H3 pre-mRNA in the Xenopus oocyte is dependent on co-injection of sea urchin U7 RNA or U7 RNP. We show that U7 RNP is a genuine member of the (Sm-type) U-snRNP family as judged by immunoprecipitation of the U7 RNA and U7 RNPs with polyclonal anti-trimethylguanosine and monoclonal anti-Sm antibodies. Sea urchin U7 RNA can be produced in the frog oocyte from RNA genes driven by a Xenopus U2 promoter. The transcripts thus synthesized have all the known characteristics of authentic U7 RNA. Our experiments further suggest that sequences near the 5' end of the U7 RNA mediate Sm-immunoprecipitability. Co-expression of sea urchin U7 RNA gene and H3 histone gene sequences in the frog oocyte complements faulty 3' maturation of histone pre-mRNA. This provides irrefutable evidence that the sea urchin U7 RNA identified earlier is indeed an essential component of the 3' processing of histone mRNAs.

PubMed Disclaimer

Cited by

mRNA Editing, Processing and Quality Control in Caenorhabditis elegans.
Arribere JA, Kuroyanagi H, Hundley HA. Arribere JA, et al. Genetics. 2020 Jul;215(3):531-568. doi: 10.1534/genetics.119.301807. Genetics. 2020. PMID: 32632025 Free PMC article. Review.
Positionally exact initiation is required for the formation of a stable RNA polymerase II transcription complex in vivo.
Tebb G, Mattaj IW. Tebb G, et al. EMBO J. 1988 Dec 1;7(12):3785-92. doi: 10.1002/j.1460-2075.1988.tb03263.x. EMBO J. 1988. PMID: 3208751 Free PMC article.
Formation of the 3' end of sea urchin U1 small nuclear RNA occurs independently of the conserved 3' box and on transcripts initiated from a histone promoter.
Wendelburg BJ, Marzluff WF. Wendelburg BJ, et al. Mol Cell Biol. 1992 Sep;12(9):4132-41. doi: 10.1128/mcb.12.9.4132-4141.1992. Mol Cell Biol. 1992. PMID: 1508209 Free PMC article.
In vivo and in vitro expression of U7 snRNA genes: cis- and trans-acting elements required for RNA polymerase II-directed transcription.
Southgate C, Busslinger M. Southgate C, et al. EMBO J. 1989 Feb;8(2):539-49. doi: 10.1002/j.1460-2075.1989.tb03408.x. EMBO J. 1989. PMID: 2721491 Free PMC article.
Purification of the individual snRNPs U1, U2, U5 and U4/U6 from HeLa cells and characterization of their protein constituents.
Bringmann P, Lührmann R. Bringmann P, et al. EMBO J. 1986 Dec 20;5(13):3509-16. doi: 10.1002/j.1460-2075.1986.tb04676.x. EMBO J. 1986. PMID: 2951249 Free PMC article.

References

1. Cell. 1977 Nov;12(3):721-32 - PubMed
1. Cell. 1985 Aug;42(1):193-202 - PubMed
1. Nature. 1980 Jan 10;283(5743):220-4 - PubMed
1. Nucleic Acids Res. 1979 Nov 10;7(5):1175-93 - PubMed
1. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5495-9 - PubMed

Genetic complementation in the Xenopus oocyte: co-expression of sea urchin histone and U7 RNAs restores 3' processing of H3 pre-mRNA in the oocyte - PubMed