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Genetic variations in the fusion protein of respiratory syncytial virus isolated from children hospitalized with community-acquired pneumonia in China - PubMed

  • ️Mon Jan 01 2018

Genetic variations in the fusion protein of respiratory syncytial virus isolated from children hospitalized with community-acquired pneumonia in China

Xiangpeng Chen et al. Sci Rep. 2018.

Abstract

To identify the variations in fusion (F) protein gene of RSV in China, a molecular epidemiological study was conducted. A total of 553 RSV positive specimens were collected from 2338 pediatric patients hospitalized with community-acquired pneumonia during a multi-center study conducted during 2014-2016. A total of 252 samples (183 RSV A, 69 RSV B) were selected for F gene sequencing, and analyzed together with 142 F gene sequences downloaded from GenBank. The result showed that all the Chinese RSV A and RSV B strains could be divided respectively into three branches. Compared with RSV A/B prototype sequences respectively, there were significant amino acid (AA) mutations at multiple antigenic sites. For RSV A, changes were found at AA residues 122, 124, 125, 276 and 384, and for RSV B at AA residues 45, 116, 125, 172, 173 and 202. Variations in human histocompatibility leukocyte antigen-restricted CTL epitopes were also observed. In total, 56 amino acid differences for the complete F protein were found between the RSV A and B groups in China, while several mutations were only found in the RSV B strains during 2015-2016. The RSV F gene is relatively conserved in China, however, limited mutations are still occurring with time.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1

The phylogenetic dendrogram based on the alignments of the 120 representative RSV F gene complete sequences was constructed with MEGA5.03 software. Panels (A and B) are detailed phylogenetic trees of the RSV A and RSV B taxa analyzed, respectively. The neighbour-joining method was used to infer the topology. The percentage of bootstrap (percentage of 1000 pseudoreplicate datasets) replicates supporting the trees are indicated at the nodes (for clarity, only values over 70% are shown). Squares indicate the prototypes RSV A Long and RSV B CH18537 strains. Filled circles indicate the strains from this study during 2014–2016. The scale bars represent substitutions per base pair per the indicated horizontal distance.

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