pubmed.ncbi.nlm.nih.gov

Interaction of distinct nuclear proteins with sequences controlling the expression of polyomavirus early genes - PubMed

Interaction of distinct nuclear proteins with sequences controlling the expression of polyomavirus early genes

E Böhnlein et al. Mol Cell Biol. 1986 May.

Abstract

The interaction between cellular factors and polyoma virus (Py) DNA was investigated by using a gel retention assay. Nuclear extracts from various cell lines (NIH 3T3, NIH 3T6, LTK-, F9) contained proteins that formed specific and distinct complexes with Py B enhancer fragments of either wild-type or F9-1 mutant origin. The presence of an excess amount of other well-characterized DNA sequences, including the Py A enhancer, the murine sarcoma virus enhancer, and the simian virus 40 enhancer-promoter region, did not interfere with this protein-DNA interaction. However, a fragment previously defined as containing the lymphotropic papovavirus enhancer shares the binding of some common factor. This observation, in combination with the results of retention gel assays at different Mg2+ concentrations, indicates the interaction of several nuclear factors and Py DNA. The assay systems that were used allowed a distinction between some factors on the basis of their different biochemical and sequence requirements. The contact sites of these complexes were mapped to the B enhancer region of Py with Bal 31-derived mutant restriction fragments and ExoIII nuclease and are compatible with the functional domains determined in vivo.

PubMed Disclaimer

Cited by

Nuclear proteins from lactating mammary glands bind to the promoter of a milk protein gene.
Lubon H, Hennighausen L. Lubon H, et al. Nucleic Acids Res. 1987 Mar 11;15(5):2103-21. doi: 10.1093/nar/15.5.2103. Nucleic Acids Res. 1987. PMID: 3031588 Free PMC article.
Activation of the polyomavirus enhancer by a murine activator protein 1 (AP1) homolog and two contiguous proteins.
Martin ME, Piette J, Yaniv M, Tang WJ, Folk WR. Martin ME, et al. Proc Natl Acad Sci U S A. 1988 Aug;85(16):5839-43. doi: 10.1073/pnas.85.16.5839. Proc Natl Acad Sci U S A. 1988. PMID: 2842750 Free PMC article.
Expression and amplification in transgenic mice of a polyoma virus mutant regulatory region.
Krippl B, Griep AE, Mahon KA, Böhnlein E, Gruss P, Westphal H. Krippl B, et al. Nucleic Acids Res. 1988 Sep 26;16(18):8963-76. doi: 10.1093/nar/16.18.8963. Nucleic Acids Res. 1988. PMID: 2845362 Free PMC article.
Purification and properties of the Rous sarcoma virus internal enhancer binding factor.
Karnitz L, Poon D, Weil PA, Chalkley R. Karnitz L, et al. Mol Cell Biol. 1989 May;9(5):1929-39. doi: 10.1128/mcb.9.5.1929-1939.1989. Mol Cell Biol. 1989. PMID: 2546054 Free PMC article.
Identification of a UV-induced trans-acting protein that stimulates polyomavirus DNA replication.
Ronai ZA, Weinstein IB. Ronai ZA, et al. J Virol. 1988 Mar;62(3):1057-60. doi: 10.1128/JVI.62.3.1057-1060.1988. J Virol. 1988. PMID: 2828653 Free PMC article.

References

1. Nature. 1985 Jun 13-19;315(6020):597-600 - PubMed
1. EMBO J. 1985 Nov;4(11):3005-13 - PubMed
1. Nature. 1980 Jan 31;283(5746):445-53 - PubMed
1. Cell. 1980 Jun;20(2):393-9 - PubMed
1. Nature. 1981 Apr 23;290(5808):720-2 - PubMed

Interaction of distinct nuclear proteins with sequences controlling the expression of polyomavirus early genes - PubMed