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Cell-cell adhesion interface: orthogonal and parallel forces from contraction, protrusion, and retraction - PubMed

  • ️Mon Jan 01 2018

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Cell-cell adhesion interface: orthogonal and parallel forces from contraction, protrusion, and retraction

Vivian W Tang. F1000Res. 2018.

Abstract

The epithelial lateral membrane plays a central role in the integration of intercellular signals and, by doing so, is a principal determinant in the emerging properties of epithelial tissues. Mechanical force, when applied to the lateral cell-cell interface, can modulate the strength of adhesion and influence intercellular dynamics. Yet the relationship between mechanical force and epithelial cell behavior is complex and not completely understood. This commentary aims to provide an investigative look at the usage of cellular forces at the epithelial cell-cell adhesion interface.

Keywords: actin; adhesion; cell-cell; contraction; epithelial; force; intercellular; lateral membrane; protrusion; retraction.

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Conflict of interest statement

No competing interests were disclosed.No competing interests were disclosed.No competing interests were disclosed.

Figures

Figure 1.
Figure 1.. Actin organization in a three-dimensional epithelial cell.

( A) Actin arrangement on the apical, lateral, and basal membranes of the epithelial cell is illustrated at the mesoscopic cellular level. ( B) Connectivity between cell–cell interface and the actin cytoskeleton is illustrated at the macroscopic multi-cellular level.

Figure 2.
Figure 2.. Direction of forces at the lateral cell–cell adhesion interface.

( A) Parallel and orthogonal forces are shown at the macroscopic multi-cellular (left panel) and mesoscopic cellular (right panel) scales. Cell–cell adhesions at the tip of a lateral membrane protrusion experience orthogonal force, whereas cell–cell adhesions at the trunk of the same membrane protrusion experience parallel force (right panel). ( B) Forces in one, two, and three dimensions can be generated through spatial organization of actin dynamics and actomyosin activities. Orthogonal and parallel one-dimensional forces can be generated with actin filaments arranged orthogonal and parallel to the plasma membrane, respectively (upper panel). Orthogonal and parallel two- and three-dimensional forces can be generated with a cross-linked actin network to support processes on the lateral cell–cell adhesion interface (lower panel).

Figure 3.
Figure 3.. Pushing and pulling to drive kinetic processes.

( A) Pulling on adhesions and membrane can break intra-molecular and inter-molecular bonds, cause movement of adhesions on the membrane, and create undulations on the cell–cell adhesion interface. ( B) Pushing parallel to the lateral membrane can disengage and disperse adhesions, resulting in mixing of the membrane components. Pushing orthogonal to the lateral membrane can increase cell–cell contact area and time to enhance adhesion engagement.

Figure 4.
Figure 4.. Pushing and pulling to create tension.

( A) Stretching the membrane increases membrane tension at the mesoscopic lateral membrane level and can enhance intercellular interactions at the microscopic molecular level. ( B) Pulling orthogonal to the membrane increases intercellular tension on the mesoscopic lateral membrane level and can enhance tension at cell–cell adhesion molecules at the microscopic molecular level. ( C) Pulling actin filament alters actin dynamics and decreases cofilin severing at the microscopic molecular level (left panel). Changing the length distribution and organization of actin filaments alters cytoskeletal tension, ultimately affecting the compressibility and rigidity of cortical actin associated with the lateral membrane at the mesoscopic lateral membrane level (middle panel). Coupling membrane and cytoskeleton tension to adhesion proteins allows integration of intercellular, membrane, cytoskeletal forces on the lateral cell–cell adhesion interface (right panel).

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