Vectors with restriction site banks. V. pJRD215, a wide-host-range cosmid vector with multiple cloning sites - PubMed

Vectors with restriction site banks. V. pJRD215, a wide-host-range cosmid vector with multiple cloning sites

J Davison et al. Gene. 1987.

Abstract

The construction of a new wide-host-range, restriction-site bank, cosmid-cloning vehicle (pJRD215) is described. The wide-host-range properties and the ability to be transferred by conjugation, extend genetic engineering to those Gram-negative species that cannot be transformed. The vector permits the cloning of genes from Gram-negative bacteria using a complementation screening procedure in a mutant host. This procedure is simplified by the possibility of construction of a cosmid gene bank so that only a few hundred clones need to be screened. Subsequent subcloning of the gene of interest is facilitated by the presence of at least 23 unique cloning sites.

Similar articles

• Stable cosmid vectors that enable the introduction of cloned fragments into a wide range of gram-negative bacteria.

  Frey J, Bagdasarian M, Feiss D, Franklin FC, Deshusses J. Frey J, et al. Gene. 1983 Oct;24(2-3):299-308. doi: 10.1016/0378-1119(83)90090-2. Gene. 1983. PMID: 6315541

• Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria.

  Keen NT, Tamaki S, Kobayashi D, Trollinger D. Keen NT, et al. Gene. 1988 Oct 15;70(1):191-7. doi: 10.1016/0378-1119(88)90117-5. Gene. 1988. PMID: 2853689

• Construction of an Actinobacillus pleuropneumoniae-Escherichia coli cosmid cloning vector [Gene 160 (1995) 81-86].

  West SE, Romero MJ. West SE, et al. Gene. 1995 Jul 4;160(1):87-8. doi: 10.1016/0378-1119(95)00225-u. Gene. 1995. PMID: 7628723

• Broad-host-range plasmid cloning vectors for gram-negative bacteria.

  Schmidhauser TJ, Ditta G, Helinski DR. Schmidhauser TJ, et al. Biotechnology. 1988;10:287-332. doi: 10.1016/b978-0-409-90042-2.50021-0. Biotechnology. 1988. PMID: 2850044 Review. No abstract available.

• [Development of a system vector-host in methylotrophic bacteria].

  Tsygankov IuD, Chistoserdov AIu, Kazakova SM. Tsygankov IuD, et al. Mol Gen Mikrobiol Virusol. 1985 Feb;(2):3-14. Mol Gen Mikrobiol Virusol. 1985. PMID: 3939573 Review. Russian.

Cited by

• Cloning and sequencing of Pseudomonas genes encoding vanillate demethylase.

  Brunel F, Davison J. Brunel F, et al. J Bacteriol. 1988 Oct;170(10):4924-30. doi: 10.1128/jb.170.10.4924-4930.1988. J Bacteriol. 1988. PMID: 3170489 Free PMC article.

• Analysis of urease expression in Actinomyces naeslundii WVU45.

  Morou-Bermudez E, Burne RA. Morou-Bermudez E, et al. Infect Immun. 2000 Dec;68(12):6670-6. doi: 10.1128/IAI.68.12.6670-6676.2000. Infect Immun. 2000. PMID: 11083780 Free PMC article.

• Amended description of the genes for synthesis of Actinomyces naeslundii T14V type 1 fimbriae and associated adhesin.

  Chen P, Cisar JO, Hess S, Ho JT, Leung KP. Chen P, et al. Infect Immun. 2007 Aug;75(8):4181-5. doi: 10.1128/IAI.01955-06. Epub 2007 May 7. Infect Immun. 2007. PMID: 17485454 Free PMC article.

• Tetracycline regulation of genes on Bacteroides conjugative transposons.

  Stevens AM, Shoemaker NB, Li LY, Salyers AA. Stevens AM, et al. J Bacteriol. 1993 Oct;175(19):6134-41. doi: 10.1128/jb.175.19.6134-6141.1993. J Bacteriol. 1993. PMID: 8407786 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database