Effect of long- and short-chain perfluorinated compounds on cultured thyroid cells viability and response to TSH - PubMed
. 2019 Nov;42(11):1329-1335.
doi: 10.1007/s40618-019-01062-1. Epub 2019 May 17.
Affiliations
- PMID: 31102255
- DOI: 10.1007/s40618-019-01062-1
Effect of long- and short-chain perfluorinated compounds on cultured thyroid cells viability and response to TSH
L Croce et al. J Endocrinol Invest. 2019 Nov.
Abstract
Purpose: Perfluorinated chemicals are widespread pollutants persistent in the environment with links to some major health issues. The two main compounds, perfluoro-octanoic acid (PFOA) and perfluoro-alkyl sulphonate (PFOS), were recently classified as carcinogenetic and thus their use has been restricted. Short-chain PFCs were recently developed as an alternative, but no data regarding the possible endocrine toxicities of these compounds are available. Aim of this study was to investigate whether short-chain PFCs could jeopardize thyroid cell viability and/or interfere with the functional effect TSH.
Methods: Fisher rat thyroid line-5 (FRTL-5) was treated with increasing concentrations of PFOA, PFOS, perfluorobutanesulfonic acid (PFBS), perfluorobutanoic acid (PFBA), pentafluoropropionic anhydride (PFPA), perfluoropentanoic acid (PFPeA) to evaluate modifications in cell viability and TSH-stimulated cAMP production.
Results: Neither long nor short-chain PFCs affected cell viability (apart from PFOS 100 µM), or interfered with cAMP production.
Conclusions: The results of the present study demonstrate for the first time that short-chain PFCs have no acute cytotoxic effect on thyroid cells in vitro and that cAMP production is not modulated by any of the tested PFCs.
Keywords: Endocrine disruptors; FRTL-5; PFOA; PFOS; Perfluorinated compounds; Thyroid.
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