No strict alignment is required between a transcriptional activator binding site and the "TATA box" of a yeast gene - PubMed
No strict alignment is required between a transcriptional activator binding site and the "TATA box" of a yeast gene
D M Ruden et al. Proc Natl Acad Sci U S A. 1988 Jun.
Abstract
GAL4 is a transcriptional activator of the galactose metabolism genes in the yeast Saccharomyces cerevisiae. We show that GAL4 expressed in yeast activated transcription equally well when a single GAL4 binding site was placed at any of nine positions upstream of the GAL1 (galactokinase gene) "TATA box." We chose a sufficient number of positions for the binding site to ensure that, in several of these positions, GAL4 was on the opposite side of the DNA helix with respect to the TATA box. Smaller GAL4 derivatives were similar to wild-type GAL4 in that they also activated transcription in a manner independent of the side of the DNA helix they bound with respect to the TATA box. Unlike wild-type GAL4, however, these smaller GAL4 derivatives activated transcription better when we placed a binding site progressively closer to the TATA box over a distance of 34 base pairs.
Similar articles
-
Selleck SB, Majors J. Selleck SB, et al. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5399-403. doi: 10.1073/pnas.85.15.5399. Proc Natl Acad Sci U S A. 1988. PMID: 3041409 Free PMC article.
-
Chen W, Struhl K. Chen W, et al. EMBO J. 1989 Jan;8(1):261-8. doi: 10.1002/j.1460-2075.1989.tb03372.x. EMBO J. 1989. PMID: 2653813 Free PMC article.
-
Inokuchi K, Nakayama A. Inokuchi K, et al. Nucleic Acids Res. 1991 Jun 11;19(11):3099-103. doi: 10.1093/nar/19.11.3099. Nucleic Acids Res. 1991. PMID: 1905400 Free PMC article.
-
A model fungal gene regulatory mechanism: the GAL genes of Saccharomyces cerevisiae.
Johnston M. Johnston M. Microbiol Rev. 1987 Dec;51(4):458-76. doi: 10.1128/mr.51.4.458-476.1987. Microbiol Rev. 1987. PMID: 2830478 Free PMC article. Review. No abstract available.
-
Transcriptional regulation in the yeast GAL gene family: a complex genetic network.
Lohr D, Venkov P, Zlatanova J. Lohr D, et al. FASEB J. 1995 Jun;9(9):777-87. doi: 10.1096/fasebj.9.9.7601342. FASEB J. 1995. PMID: 7601342 Review.
Cited by
-
GAL4 is regulated by a glucose-responsive functional domain.
Stone G, Sadowski I. Stone G, et al. EMBO J. 1993 Apr;12(4):1375-85. doi: 10.1002/j.1460-2075.1993.tb05782.x. EMBO J. 1993. PMID: 8467796 Free PMC article.
-
Finley RL Jr, Chen S, Ma J, Byrne P, West RW Jr. Finley RL Jr, et al. Mol Cell Biol. 1990 Nov;10(11):5663-70. doi: 10.1128/mcb.10.11.5663-5670.1990. Mol Cell Biol. 1990. PMID: 2122231 Free PMC article.
-
Müller-Immerglück MM, Schaffner W, Matthias P. Müller-Immerglück MM, et al. EMBO J. 1990 May;9(5):1625-34. doi: 10.1002/j.1460-2075.1990.tb08282.x. EMBO J. 1990. PMID: 2328728 Free PMC article.
-
Watanabe K, Kokubo T. Watanabe K, et al. PLoS One. 2017 Nov 27;12(11):e0188435. doi: 10.1371/journal.pone.0188435. eCollection 2017. PLoS One. 2017. PMID: 29176831 Free PMC article.
-
The initiator directs the assembly of a transcription factor IID-dependent transcription complex.
Carcamo J, Buckbinder L, Reinberg D. Carcamo J, et al. Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):8052-6. doi: 10.1073/pnas.88.18.8052. Proc Natl Acad Sci U S A. 1991. PMID: 1896450 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials