Structural and functional characterization of the short acidic transcriptional activation region of yeast GCN4 protein - PubMed

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• PMID: 3287180
• DOI: 10.1038/333635a0

Structural and functional characterization of the short acidic transcriptional activation region of yeast GCN4 protein

I A Hope et al. Nature. 1988.

Abstract

Derivatives of the yeast GCN4 transcription factor containing acidic regions of 35 to 40 amino acids fused directly to the DNA-binding domain are fully functional in vivo. High resolution deletion analysis and proteolytic mapping suggest that the activation region is a repeated structure composed of small units acting additively. Acidic character is a feature of the structural motif, possibly a dimer of alpha-helices from two GCN4 monomers, that may be important for interactions with the basic transcriptional machinery.

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