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Evolution and mutagenesis of the mammalian excision repair gene ERCC-1 - PubMed

  • ️Fri Jan 01 1988

Comparative Study

. 1988 Jun 24;16(12):5305-22.

doi: 10.1093/nar/16.12.5305.

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Free PMC article

Comparative Study

Evolution and mutagenesis of the mammalian excision repair gene ERCC-1

M van Duin et al. Nucleic Acids Res. 1988.

Free PMC article

Abstract

The human DNA excision repair protein ERCC-1 exhibits homology to the yeast RAD10 repair protein and its longer C-terminus displays similarity to parts of the E. coli repair proteins uvrA and uvrC. To study the evolution of this 'mosaic' ERCC-1 gene we have isolated the mouse homologue. Mouse ERCC-1 harbors the same pattern of homology with RAD10 and has a comparable C-terminal extension as its human equivalent. Mutation studies show that the strongly conserved C-terminus is essential in contrast to the less conserved N-terminus which is even dispensible. The mouse ERCC-1 amino acid sequence is compatible with a previously postulated nuclear location signal and DNA-binding domain. The ERCC-1 promoter harbors a region which is highly conserved in mouse and man. Since the ERCC-1 promoter is devoid of all classical promoter elements this region may be responsible for the low constitutive level of expression in all mouse tissues and stages of embryogenesis examined.

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