Study of yeast mitochondrial tRNAs by two-dimensional polyacrylamide gel electrophoresis: characterization of isoaccepting species and search for imported cytoplasmic tRNAs - PubMed

Study of yeast mitochondrial tRNAs by two-dimensional polyacrylamide gel electrophoresis: characterization of isoaccepting species and search for imported cytoplasmic tRNAs

R P Martin et al. Nucleic Acids Res. 1977 Oct.

Free PMC article

Abstract

By two-dimensional polyacrylamide gel electrophoresis, yeast mitochondrial tRNA is fractionated into 27 major species. All but 6 of them migrate distinctly from cytoplasmic tRNAs. Migration of mitochondrial DNA-coded mitochondrial tRNAs shows the occurence of only one cytoplasmic tRNA in mitochondria. Several mitochondrial tRNA spots are identified on the electrophoregrams, some of them show isoaccepting species (Val, Ser, Met, Leu). It is suggested that there are sufficient mitochondrial tRNA genes on yeast mitochondrial DNA to allow mitochondrial protein biosynthesis by the mitochondrial tRNAs alone. Guanosine + Cytidine content and rate base composition are reported for some individual species. Mitochondrial tRNAPhe lacks Ribothymidine.

Similar articles

• Two dimensional polyacrylamide gel electrophoresis analysis of Tetrahymena mitochondrial tRNA.

  Suyama Y. Suyama Y. Curr Genet. 1986;10(5):411-20. doi: 10.1007/BF00418415. Curr Genet. 1986. PMID: 3127061

• Number of genes and base composition of mitochondrial tRNA from Saccharomyces cerevisiae.

  Schneller JM, Faye G, Kujawa C, Stahl AJ. Schneller JM, et al. Nucleic Acids Res. 1975 Jun;2(6):831-8. doi: 10.1093/nar/2.6.831. Nucleic Acids Res. 1975. PMID: 1096083 Free PMC article.

• Coding origin of isoaccepting tRNA in yeast mitochondria.

  Schneller JM, Stahl A, Fukuhara H. Schneller JM, et al. Biochimie. 1975;57(9):1051-7. doi: 10.1016/s0300-9084(75)80361-0. Biochimie. 1975. PMID: 769848

• Mitochondrial tRNA import and its consequences for mitochondrial translation.

  Schneider A. Schneider A. Annu Rev Biochem. 2011;80:1033-53. doi: 10.1146/annurev-biochem-060109-092838. Annu Rev Biochem. 2011. PMID: 21417719 Review.

• Transfer RNA travels from the cytoplasm to organelles.

  Rubio MA, Hopper AK. Rubio MA, et al. Wiley Interdiscip Rev RNA. 2011 Nov-Dec;2(6):802-17. doi: 10.1002/wrna.93. Epub 2011 Jul 11. Wiley Interdiscip Rev RNA. 2011. PMID: 21976284 Free PMC article. Review.

Cited by

• Mitochondrially-imported cytoplasmic tRNA(Lys)(CUU) of Saccharomyces cerevisiae: in vivo and in vitro targetting systems.

  Tarassov IA, Entelis NS. Tarassov IA, et al. Nucleic Acids Res. 1992 Mar 25;20(6):1277-81. doi: 10.1093/nar/20.6.1277. Nucleic Acids Res. 1992. PMID: 1561084 Free PMC article.

• The mitochondrial genome of Paraspadella gotoi is highly reduced and reveals that chaetognaths are a sister group to protostomes.

  Helfenbein KG, Fourcade HM, Vanjani RG, Boore JL. Helfenbein KG, et al. Proc Natl Acad Sci U S A. 2004 Jul 20;101(29):10639-43. doi: 10.1073/pnas.0400941101. Epub 2004 Jul 12. Proc Natl Acad Sci U S A. 2004. PMID: 15249679 Free PMC article.

• Translation initiation in mammalian mitochondria- a prokaryotic perspective.

  Ayyub SA, Varshney U. Ayyub SA, et al. RNA Biol. 2020 Feb;17(2):165-175. doi: 10.1080/15476286.2019.1690099. Epub 2019 Nov 14. RNA Biol. 2020. PMID: 31696767 Free PMC article. Review.

• MboI, ThaI and HinfI endonuclease cleavage maps of the yeast mitochondrial DNA : Localization of transfer RNA genes.

  Wésolowski M, Monnerot M, Fukuhara H. Wésolowski M, et al. Curr Genet. 1980 Oct;2(2):121-9. doi: 10.1007/BF00420624. Curr Genet. 1980. PMID: 24189803

• Mitochondrial import of a cytoplasmic lysine-tRNA in yeast is mediated by cooperation of cytoplasmic and mitochondrial lysyl-tRNA synthetases.

  Tarassov I, Entelis N, Martin RP. Tarassov I, et al. EMBO J. 1995 Jul 17;14(14):3461-71. doi: 10.1002/j.1460-2075.1995.tb07352.x. EMBO J. 1995. PMID: 7628447 Free PMC article.

References

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • PubMed Central
  • Silverchair Information Systems

• Miscellaneous

  • NCI CPTAC Assay Portal