Sliding of STOP proteins on microtubules: a model system for diffusion-dependent microtubule motility - PubMed

Sliding of STOP proteins on microtubules: a model system for diffusion-dependent microtubule motility

R L Margolis et al. Ann N Y Acad Sci. 1986.

Abstract

STOP proteins, of 145 kD, act substoichiometrically to block end-wise disassembly of microtubules. STOPs bind to microtubules either during microtubule assembly or when added at steady state, and when binding to the polymers is apparently irreversible. They are not measurably lost from polymers under competition conditions, and there is no measurable exchange between polymers. Nonetheless, STOP proteins exhibit an extraordinary behavior: they "slide" laterally on the surface of the microtubule. Displacement is assayed by forming hybrid microtubules in which cold stable or cold labile region subunits are labeled. Displacement of STOPs on the polymer with time will cause labeled subunits of cold-stable regions to become increasingly cold labile in a manner reciprocal to cold stabilization of previously cold-labile subunits. Because equilibrium exchange of STOP proteins onto and off the polymers can be ruled out, the displacement of STOPs relative to subunits can only be explained by lateral diffusion or "sliding." Axonal transport and mitotic mechanisms were discussed as implications of such a lateral translocation mechanism for microtubule-dependent motility.

Similar articles

• Sliding of STOP proteins on microtubules.

  Pabion M, Job D, Margolis RL. Pabion M, et al. Biochemistry. 1984 Dec 18;23(26):6642-8. doi: 10.1021/bi00321a055. Biochemistry. 1984. PMID: 6529574

• Isolation from bovine brain of a superstable microtubule subpopulation with microtubule seeding activity.

  Job D, Margolis RL. Job D, et al. Biochemistry. 1984 Jun 19;23(13):3025-31. doi: 10.1021/bi00308a028. Biochemistry. 1984. PMID: 6466629

• Generation of microtubule stability subclasses by microtubule-associated proteins: implications for the microtubule "dynamic instability" model.

  Job D, Pabion M, Margolis RL. Job D, et al. J Cell Biol. 1985 Nov;101(5 Pt 1):1680-9. doi: 10.1083/jcb.101.5.1680. J Cell Biol. 1985. PMID: 4055892 Free PMC article.

• Role of microtubule-associated proteins in the control of microtubule assembly.

  Maccioni RB, Cambiazo V. Maccioni RB, et al. Physiol Rev. 1995 Oct;75(4):835-64. doi: 10.1152/physrev.1995.75.4.835. Physiol Rev. 1995. PMID: 7480164 Review.

• Cold-stable and cold-adapted microtubules.

  Wallin M, Strömberg E. Wallin M, et al. Int Rev Cytol. 1995;157:1-31. doi: 10.1016/s0074-7696(08)62155-5. Int Rev Cytol. 1995. PMID: 7706018 Review.

Cited by

• A 17-kD centromere protein (CENP-A) copurifies with nucleosome core particles and with histones.

  Palmer DK, O'Day K, Wener MH, Andrews BS, Margolis RL. Palmer DK, et al. J Cell Biol. 1987 Apr;104(4):805-15. doi: 10.1083/jcb.104.4.805. J Cell Biol. 1987. PMID: 3558482 Free PMC article.

• Specific association of STOP protein with microtubules in vitro and with stable microtubules in mitotic spindles of cultured cells.

  Margolis RL, Rauch CT, Pirollet F, Job D. Margolis RL, et al. EMBO J. 1990 Dec;9(12):4095-102. doi: 10.1002/j.1460-2075.1990.tb07631.x. EMBO J. 1990. PMID: 2249667 Free PMC article.

• Model of anaphase chromosome movement based on polymer-guided diffusion.

  Garel JR, Job D, Margolis RL. Garel JR, et al. Proc Natl Acad Sci U S A. 1987 Jun;84(11):3599-603. doi: 10.1073/pnas.84.11.3599. Proc Natl Acad Sci U S A. 1987. PMID: 3473470 Free PMC article.

• Tau: It's Not What You Think.

  Baas PW, Qiang L. Baas PW, et al. Trends Cell Biol. 2019 Jun;29(6):452-461. doi: 10.1016/j.tcb.2019.02.007. Epub 2019 Mar 28. Trends Cell Biol. 2019. PMID: 30929793 Free PMC article. Review.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Wiley