Nucleolin, the major nucleolar protein of growing eukaryotic cells: an unusual protein structure revealed by the nucleotide sequence - PubMed

Nucleolin, the major nucleolar protein of growing eukaryotic cells: an unusual protein structure revealed by the nucleotide sequence

B Lapeyre et al. Proc Natl Acad Sci U S A. 1987 Mar.

Abstract

Nucleolin (also called C23) is the major nucleolar protein of exponentially growing eukaryotic cells. It is found associated with intranucleolar chromatin and preribosomal particles. Through use of a polyclonal antiserum, nucleolin cDNA clones were isolated from a Chinese hamster ovary cell library constructed in the expression vector lambda gt11. The isolated cDNAs encoded a polypeptide containing 679 residues of the 713 amino acids of nucleolin. The amino acid sequence presents several unusual features: in particular, repetitive sequences are found at both ends of the molecule. A repeat, Hy-Thr-Pro-Hy-Lys-Lys-Hy-Hy, in which Hy is a nonpolar residue, is found six times in the NH2-end proximal portion, followed by three acidic stretches containing 25, 25, and 33 glutamic acid or aspartic acid residues. Four potential phosphorylation sites (serines) are also observed in this region. The COOH-terminal proximal portion of the protein carries a glycine-rich region with fairly regularly interspersed phenylalanine and dimethylarginine residues. The two terminal portions of the molecule exhibit unique potential secondary structures: alpha-helix (NH2 terminus) and extended (COOH terminus). The central region exhibits alternating hydrophobic and hydrophilic stretches. Five potential N glycosylation sites are detected. The structure of this protein may reflect two functions in preribosome biogenesis: interaction with chromatin (NH2 terminus) and with preribosomes (COOH terminus).

Similar articles

• Protein and cDNA sequence of a glycine-rich, dimethylarginine-containing region located near the carboxyl-terminal end of nucleolin (C23 and 100 kDa).

  Lapeyre B, Amalric F, Ghaffari SH, Rao SV, Dumbar TS, Olson MO. Lapeyre B, et al. J Biol Chem. 1986 Jul 15;261(20):9167-73. J Biol Chem. 1986. PMID: 3755137

• Phosphorylation of nucleolin by a nucleolar type NII protein kinase.

  Caizergues-Ferrer M, Belenguer P, Lapeyre B, Amalric F, Wallace MO, Olson MO. Caizergues-Ferrer M, et al. Biochemistry. 1987 Dec 1;26(24):7876-83. doi: 10.1021/bi00398a051. Biochemistry. 1987. PMID: 3427111

• The glycine-rich domain of nucleolin has an unusual supersecondary structure responsible for its RNA-helix-destabilizing properties.

  Ghisolfi L, Joseph G, Amalric F, Erard M. Ghisolfi L, et al. J Biol Chem. 1992 Feb 15;267(5):2955-9. J Biol Chem. 1992. PMID: 1737751

• Cloning and sequencing of the human nucleolin cDNA.

  Srivastava M, Fleming PJ, Pollard HB, Burns AL. Srivastava M, et al. FEBS Lett. 1989 Jun 19;250(1):99-105. doi: 10.1016/0014-5793(89)80692-1. FEBS Lett. 1989. PMID: 2737305

• Molecular dissection of nucleolin's role in growth and cell proliferation: new insights.

  Srivastava M, Pollard HB. Srivastava M, et al. FASEB J. 1999 Nov;13(14):1911-22. FASEB J. 1999. PMID: 10544174 Review.

Cited by

• A novel post-translational modification of nucleolin, SUMOylation at Lys-294, mediates arsenite-induced cell death by regulating gadd45α mRNA stability.

  Zhang D, Liang Y, Xie Q, Gao G, Wei J, Huang H, Li J, Gao J, Huang C. Zhang D, et al. J Biol Chem. 2015 Feb 20;290(8):4784-4800. doi: 10.1074/jbc.M114.598219. Epub 2015 Jan 5. J Biol Chem. 2015. PMID: 25561743 Free PMC article.

• 'SPKK' motifs prefer to bind to DNA at A/T-rich sites.

  Churchill ME, Suzuki M. Churchill ME, et al. EMBO J. 1989 Dec 20;8(13):4189-95. doi: 10.1002/j.1460-2075.1989.tb08604.x. EMBO J. 1989. PMID: 2556263 Free PMC article.

• Multiple domains of the large fibroblast proteoglycan, versican.

  Zimmermann DR, Ruoslahti E. Zimmermann DR, et al. EMBO J. 1989 Oct;8(10):2975-81. doi: 10.1002/j.1460-2075.1989.tb08447.x. EMBO J. 1989. PMID: 2583089 Free PMC article.

• The Ag-NOR proteins and transcription and duplication of ribosomal genes in mammalian cell nucleoli.

  Pession A, Farabegoli F, Treré D, Novello F, Montanaro L, Sperti S, Rambelli F, Derenzini M. Pession A, et al. Chromosoma. 1991 May;100(4):242-50. doi: 10.1007/BF00344158. Chromosoma. 1991. PMID: 1711437

• RNP-T, a ribonucleoprotein from Arabidopsis thaliana, contains two RNP-80 motifs and a novel acidic repeat arranged in an alpha-helix conformation.

  Bar-Zvi D, Shagan T, Schindler U, Cashmore AR. Bar-Zvi D, et al. Plant Mol Biol. 1992 Dec;20(5):833-8. doi: 10.1007/BF00027154. Plant Mol Biol. 1992. PMID: 1463823

References

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations Database