pubmed.ncbi.nlm.nih.gov

Effect of protein kinase P on phosphorylations catalyzed by the epidermal growth factor - PubMed

Effect of protein kinase P on phosphorylations catalyzed by the epidermal growth factor

M Abdel-Ghany et al. Proc Natl Acad Sci U S A. 1987 Dec.

Abstract

Protein kinase P (PK-P) activated by histones or certain other basic compounds has been purified previously from yeast [Yanagita, Y., Abdel-Ghany, M., Raden, D., Nelson, N. & Racker, E. (1987) Proc. Natl. Acad. Sci. USA 84, 925-929]. It is shown here that PK-P is present in solubilized membranes of A-431 carcinoma cells where it changes the epidermal growth factor (EGF) receptor kinase activity. Polylysine, a weak PK-P activator, inhibited the autophosphorylation of the EGF receptor both in the absence and presence of EGF. Increased PK-P activity induced by histone 1, a potent activator, gave rise to increased autophosphorylation of the EGF receptor as well as phosphorylation at tyrosine residues of numerous other endogenous membrane components. The stimulation by histone was particularly striking in the presence of EGF. A similar stimulation was achieved with polylysine and EGF on addition of yeast PK-P. However, addition of yeast PK-P in the presence of histone 1 markedly inhibited the EGF-stimulated phosphorylation of endogenous membrane proteins. We conclude from these results that the effect of PK-P on the EGF receptor takes place in three phases: at low levels PK-P inhibits the autophosphorylation, at intermediate levels it stimulates the autophosphorylation as well as the EGF-dependent phosphorylation of numerous other membrane proteins, and at high levels it inhibits the phosphorylation of these proteins.

PubMed Disclaimer

Cited by

Heparin stimulates epidermal growth factor receptor-mediated phosphorylation of tyrosine and threonine residues.
Revis-Gupta S, Abdel-Ghany M, Koland J, Racker E. Revis-Gupta S, et al. Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):5954-8. doi: 10.1073/pnas.88.14.5954. Proc Natl Acad Sci U S A. 1991. PMID: 1712476 Free PMC article.
Phosphorylation of the RAS2 gene product by protein kinase A inhibits the activation of yeast adenylyl cyclase.
Resnick RJ, Racker E. Resnick RJ, et al. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2474-8. doi: 10.1073/pnas.85.8.2474. Proc Natl Acad Sci U S A. 1988. PMID: 3128788 Free PMC article.
Brain protein kinase C phosphorylating poly(arginine,serine) or lamin B is stimulated by anions and by an activator purified from bovine serum albumin preparations.
Abdel-Ghany M, el-Gendy K, Zhang S, Raden D, Racker E. Abdel-Ghany M, et al. Proc Natl Acad Sci U S A. 1989 Mar;86(6):1761-5. doi: 10.1073/pnas.86.6.1761. Proc Natl Acad Sci U S A. 1989. PMID: 2928301 Free PMC article.
Phosphorylation of synthetic random polypeptides by protein kinase P and other protein-serine (threonine) kinases and stimulation or inhibition of kinase activities by microbial toxins.
Abdel-Ghany M, Raden D, Racker E, Katchalski-Katzir E. Abdel-Ghany M, et al. Proc Natl Acad Sci U S A. 1988 Mar;85(5):1408-11. doi: 10.1073/pnas.85.5.1408. Proc Natl Acad Sci U S A. 1988. PMID: 3125547 Free PMC article.
Stimulation of phosphorylation of lipocortin at threonine residues by epidermal growth factor (EGF) and the EGF receptor: addition of protein kinase P with polylysine inhibits this effect.
Abdel-Ghany M, Kole HK, el Saad MA, Racker E. Abdel-Ghany M, et al. Proc Natl Acad Sci U S A. 1989 Aug;86(16):6072-6. doi: 10.1073/pnas.86.16.6072. Proc Natl Acad Sci U S A. 1989. PMID: 2548197 Free PMC article.

References

1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
1. Mol Cell Biol. 1981 Feb;1(2):165-78 - PubMed
1. Biochem J. 1977 Nov 15;168(2):187-94 - PubMed
1. Cell. 1981 Jun;24(3):741-52 - PubMed
1. Biochemistry. 1982 Nov 23;21(24):6138-44 - PubMed

Effect of protein kinase P on phosphorylations catalyzed by the epidermal growth factor - PubMed