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The Anti-Adiposity Mechanisms of Ampelopsin and Vine Tea Extract in High Fat Diet and Alcohol-Induced Fatty Liver Mouse Models - PubMed

  • ️Sat Jan 01 2022

The Anti-Adiposity Mechanisms of Ampelopsin and Vine Tea Extract in High Fat Diet and Alcohol-Induced Fatty Liver Mouse Models

Jianbo Wu et al. Molecules. 2022.

Abstract

Ampelopsis grossedentata (AG) is an ancient medicinal plant that is mainly distributed and used in southwest China. It exerts therapeutic effects, such as antioxidant, anti-diabetic, and anti-inflammatory activities, reductions in blood pressure and cholesterol and hepatoprotective effects. Researchers in China recently reported the anti-obesity effects of AG extract in diet-induced obese mice and rats. To verify these findings, we herein investigated the effects of AG extract and its principal compound, ampelopsin, in high-fat diet (HFD)- and alcohol diet-fed mice, olive oil-loaded mice, and differentiated 3T3-L1 cells. The results obtained showed that AG extract and ampelopsin significantly suppressed increases in the weights of body, livers and abdominal fat and also up-regulated the expression of carnitine palmitoyltransferase 1A in HFD-fed mice. In olive oil-loaded mice, AG extract and ampelopsin significantly attenuated increases in serum triglyceride (TG) levels. In differentiated 3T3-L1 cells, AG extract and ampelopsin promoted TG decomposition, which appeared to be attributed to the expression of hormone-sensitive lipase. In alcohol diet-fed mice, AG extract and ampelopsin reduced serum levels of ethanol, glutamic oxaloacetic transaminase (GOT), and glutamic pyruvic transaminase (GPT) and liver TG. An examination of metabolic enzyme expression patterns revealed that AG extract and ampelopsin mainly enhanced the expression of aldehyde dehydrogenase and suppressed that of cytochrome P450, family 2, subfamily e1. In conclusion, AG extract and ampelopsin suppressed diet-induced intestinal fat accumulation and reduced the risk of fatty liver associated with HFD and alcohol consumption.

Keywords: Ampelopsis grossedentata; abdominal fat; ampelopsin; fatty liver; obesity; vine tea.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1

HPLC chromatogram of AG extract.

Figure 2
Figure 2

Effects of AG extract and ampelopsin on HFD-induced body weight changes in mice. Each symbol represents the mean ± SE (n = 7). Data were assessed by a one-way analysis (ANOVA) of variance followed by Dunnett’s method. Asterisks denote significant differences from the control group at * p < 0.05, ** p < 0.01.

Figure 3
Figure 3

Effects of AG extract and ampelopsin on CPT1A protein expression in the liver of HFD-fed mice. Each column represents the mean ± SE (n = 3–4). Data were assessed by a one-way analysis (ANOVA) of variance followed by Dunnett’s method. Asterisks denote significant differences from the control group at * p < 0.05.

Figure 4
Figure 4

Effects of AG extract and ampelopsin on lipid absorption in olive oil-loaded mice. Each symbol represents the mean ± SE (n = 6). Data were assessed by a one-way analysis (ANOVA) of variance followed by Dunnett’s method. Asterisks denote significant differences from the control group at * p < 0.05, ** p < 0.01.

Figure 5
Figure 5

Effects of AG extract and ampelopsin on lipolysis in differentiated 3T3-L1 adipocytes. Each column represents the mean ± SE (n = 2–3).

Figure 6
Figure 6

Effects of AG extract and ampelopsin on serum ethanol, GOT and GPT in alcohol diet-fed mice. Each column represents the mean ± SE (n = 5–6). Data were assessed by a one-way analysis (ANOVA) of variance followed by Dunnett’s method. Asterisks denote significant differences from the control group at * p < 0.05, ** p < 0.01.

Figure 7
Figure 7

Effects of AG extract and ampelopsin on liver TG and microscopic images of hematoxylin-eosin staining. Each column represents the mean ± SE (n = 4–5). Data were assessed by a one-way analysis (ANOVA) of variance followed by Dunnett’s method. Asterisks denote significant differences from the control group at * p < 0.05, ** p < 0.01.

Figure 8
Figure 8

Effects of AG extract and ampelopsin on ALDH2 and CYP2E1 protein expression in the liver of alcohol diet-fed mice. Each column represents the mean ± SE (n = 3–4). Data were assessed by a one-way analysis (ANOVA) of variance followed by Dunnett’s method. Asterisks denote significant differences from the control group at * p < 0.05, ** p < 0.01.

Figure 9
Figure 9

Potential underlying pathway by which AG extract and ampelopsin inhibits lipid absorption and fat accumulation and promotes fat decomposition.

Figure 10
Figure 10

Potential underlying pathway by which AG extract and ampelopsin contribute to ethanol metabolism in the liver.

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