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Special Thai Oolong Tea: Chemical Profile and In Vitro Antidiabetic Activities - PubMed

  • ️Sat Jan 01 2022

Special Thai Oolong Tea: Chemical Profile and In Vitro Antidiabetic Activities

Narawadee Rujanapun et al. Front Pharmacol. 2022.

Abstract

Special Thai oolong tea is oolong tea (Camellia sinensis (L.) Kuntze) steamed with selected Thai botanical drugs. Oolong tea steamed with ginger (Zingiber officinale), lemongrass (Cymbopogon citratus), and celery (Anathallis graveolens L.) is called eternity tea (EN), whereas peaceful rest (PR) tea is made of oolong tea leaves steamed with Indian gooseberry (Phyllanthus emblica), Turkey berry (Solanum torvum), and wild betel leaf bush leaves (Piper sarmentosum). Oolong tea is known for its numerous biological activities including antidiabetic properties. However, the effect of the additional botanical drugs on the biological activities of special oolong teas has not yet been explored. From the results, the PR extract exhibited the best activity in the in vitro assays relevant to antidiabetic properties such as chemical antioxidant, anti-inflammation, anti-adipogenesis, enzyme inhibition, and glucose uptake and consumption. The UHPLC-QTOF-MS/MS profiles of PR and EN extracts indicated chemical profiles different from oolong tea. For instance, gingerdiol and gingerol were detected in EN, whereas piperettine I was detected in PR. Therefore, it was inferred that among the three tea extracts, the additional compounds in PR contributed to good activities compared to oolong and EN. It is also important to highlight that the PR extract inhibited glucose uptake and consumption by adipocytes and skeletal muscles at concentrations of 500 and 100 μg/ml, respectively, as well as metformin activity (p < 0.05). Findings from this study support the antidiabetic potential of PR tea.

Keywords: Thai botanical drug; antidiabetic; eternity tea; hyperglycemia; oolong teas; peaceful rest tea.

Copyright © 2022 Rujanapun, Jaidee, Duangyod, Phuneerub, Paojumroom, Maneerat, Pringpuangkeo, Ramli and Charoensup.

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Conflict of interest statement

Author CP was employed by company Doi Chang Tea Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1

Effect of tea water extracts on glucose consumption in 3T3-L1 cells. PR, peaceful rest tea; EN, eternity tea.

FIGURE 2
FIGURE 2

Effect of PR, EN, and oolong tea water extracts at concentration of 100 μg/ml on glucose uptake in L6 cells. PR, peaceful rest tea; EN, eternity tea.

FIGURE 3
FIGURE 3

Effect of tea water extracts (50 μg/ml) on intracellular lipid accumulation. (A) Lipid droplets of adipocyte cells after red oil O staining. (B) Percentage of relative lipid content in adipocyte cells after tea water treatment. Arrows indicate lipid droplets. PR, peaceful rest tea; EN, eternity tea.

FIGURE 4
FIGURE 4

Chemical profile of tea extracts. (A) Total ion chromatogram of oolong, EN, and PR. (B) Score plot PCA of the chemical compounds from oolong, EN, and PR. RT, retention time; PR, peaceful rest tea; EN, eternity tea.

FIGURE 5
FIGURE 5

Effect of tea water extracts on RAW 264.7 cells. (A) Cell viability percentage. (B) Percentage of nitric oxide production in RAW cells after tea extract treatment. PR, peaceful rest tea; EN, eternity tea; NO production, nitric oxide production.

FIGURE 6
FIGURE 6

Anti-proliferation effect of tea extracts on K562 cells. PR, peaceful rest tea; EN, eternity tea.

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