pubmed.ncbi.nlm.nih.gov

Neuroprotective potential of terpenoid-rich extracts from orange juice by-products obtained by pressurized liquid extraction - PubMed

  • ️Sat Jan 01 2022

Neuroprotective potential of terpenoid-rich extracts from orange juice by-products obtained by pressurized liquid extraction

José David Sánchez-Martínez et al. Food Chem X. 2022.

Abstract

Pressurized liquid extraction (PLE) conditions were optimized to improve the recovery of orange (Citrus sinensis) by-products terpenoids. The neuroprotective potential of the PLE extracts were tested against a set of in-vitro assay (antioxidant (ABTS), reactive oxygen/nitrogen species (ROS/RNS)) as well as enzymatic tests (acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and lipoxygenase (LOX)). Gas chromatography coupled to high-resolution mass spectrometry (GC-q-TOF-MS) analysis revealed a higher enrichment in mono- and sesquiterpenoids of the PLE extracts with the highest neuroprotection capacity. In-silico molecular docking analysis showed the specific interaction of representative terpenoids with enzymes active sites. The results demonstrate that the selected extract at 100 °C and 30 minutes possesses high antioxidant (ABTSIC50 = 13.5 μg mL-1; ROSIC50 = 4.4 μg mL-1), anti-cholinesterase (AChEIC50 = 137.1 vg L-1; BChEIC50 = 147.0 μg mL-1) and anti-inflammatory properties (against IL-6 and LOXIC50 = 76.1 μg mL-1), with low cytotoxicity and protection against L-glutamic acid in cell models.

Keywords: Anti-inflammatory; GC-q-TOF-MS; Green-extraction; Neuroprotective; Orange juice by-products; Pressurized liquid extraction; Terpenoids.

© 2022 The Authors.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1

Heat map from Orange PLE extract and their terpenoid composition.

Fig. 2
Fig. 2

PCA projection of PLE orange extracts and studied variables.

Fig. 3
Fig. 3

PCA projection of PLE orange extracts and studied variablesFigure 3. a Effect of different concentrations of PLE100 extract on cell viability in HK-2, differentiated THP-1 and SHSY5Y cells. Fig. 3b. Secretion levels of TNF-α, IL-6 and IL-1β in differentiated THP-1 cells treated with and without LPS treatment (Control + and Control -, respectively), and treated with LPS in the presence of PLE100 extract 30, 60 and 120 μg mL−1. Each bar is the mean of three determinations ± SD. * Denotes statistical differences when compared PLE100-treated cells with positive control (*: p < 0.05; **: p < 0.01). Fig. 3c. Neuroprotective effect of pre-incubation of PLE100 extract against the neurotoxic agents

l

-glutamate (23 mM) and Aβ1-42 (30 µM) in differentiated SH-SY5Y cells. Non Aβ1-42-treated cells were used as Control, together with only PLE100-treated cells (PLE100) at 30 μg mL−1. The results are mean ± SD. * Denotes statistical differences between Control and PLE100 when neurotoxic agent is added (*: p < 0.05).

Similar articles

Cited by

References

    1. Ademosun A.O., Oboh G. Anticholinesterase and antioxidative properties of water-extractable phytochemicals from some citrus peels. Journal of Basic and Clinical Physiology and Pharmacology. 2014;25(2):199–204. doi: 10.1515/jbcpp-2013-0027. - DOI - PubMed
    1. AlFadly E.D., Elzahhar P.A., Tramarin A., Elkazaz S., Shaltout H., Abu-Serie M.M.…Belal A.S.F. Tackling neuroinflammation and cholinergic deficit in Alzheimer’s disease: Multi-target inhibitors of cholinesterases, cyclooxygenase-2 and 15-lipoxygenase. European Journal of Medicinal Chemistry. 2019;167:161–186. doi: 10.1016/j.ejmech.2019.02.012. - DOI - PubMed
    1. Alvarez-Rivera G., Bueno M., Ballesteros-Vivas D., Mendiola J.A., Ibañez E. Liquid-Phase Extraction. Elsevier; 2020. Pressurized Liquid Extraction; pp. 375–398. - DOI
    1. Armstrong, S. R. D. (2016). Oxidative Stress and Antioxidant Protection.
    1. Atolani O., Adamu N., Oguntoye O.S., Zubair M.F., Fabiyi O.A., Oyegoke R.A.…Olatunji G.A. Chemical characterization, antioxidant, cytotoxicity, Anti-Toxoplasma gondii and antimicrobial potentials of the Citrus sinensis seed oil for sustainable cosmeceutical production. Heliyon. 2020;6(2):e03399. doi: 10.1016/j.heliyon.2020.e03399. - DOI - PMC - PubMed

LinkOut - more resources