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Potent Bioactivity of Endophytic Fungi Isolated from Moringa oleifera Leaves - PubMed

  • ️Sat Jan 01 2022

Potent Bioactivity of Endophytic Fungi Isolated from Moringa oleifera Leaves

Bushra Rehman et al. Biomed Res Int. 2022.

Abstract

Plant species are known to harbor large number of endophytes, which stays in plant tissues as symbionts. These endophytes secrete large array of bioactive compounds that have potency against certain diseases with no side effects. We have collected leaf samples of the Moringa oleifera plant from the Pakistan Forest Institute, Khyber Pakhtunkhwa, Pakistan for the isolation of beneficial endophytes. The strains isolated from the leaves of M. oleifera were coded with MOL and tested for antimicrobial, antifungal, germicidal, phytotoxic, insecticidal, cytotoxic, and anti-inflammatory activities. The isolates, MOL1, MOL16, MOL19, and MOL21, possessed antibacterial activity against Staphylococcus aureus, whereas MOL7 inhibited 55% of the growth of Escherichia coli. MOL3 inhibited the growth of E. coli, S. aureus, and Pseudomonas aeruginosa. The strains, MOL1 and MOL7, showed antifungal activity against Candida albicans and Saccharomyces cerevisiae, while the strains, MOL11 and MOL17, showed activity against Verticillium chlamydosporium. The isolates, MOL3, MOL7, MOL9, MOL15, MOL17, MOL18, and MOL19, inhibited the growth of Lemna minor (duckweed) at 100 μg/ml. MOL2 exhibited strong activity in the brine shrimp assay, while MOL1, MOL2, MOL5, MOL6, MOL12, MOL17, MOL19, and MOL20 showed insecticidal, and MOL3 demonstrated larvicidal and antileishmanial activity. The isolated potent endophytes were identified as Aspergillus, Penicillium, Fusarium, Tricoderma, Rhizoctonia, Mucor, Alternaria, Pestalotiopsis, Acremonium, and Cladosporium through morphological and microscopic characteristics of the colonies.

Copyright © 2022 Bushra Rehman et al.

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Conflict of interest statement

The authors declare that there is no competing interest of any nature related to this manuscript.

Figures

Figure 1
Figure 1

Antibacterial activity of endophytes isolated from M. oleifera leaves.

Figure 2
Figure 2

Antifungal activity of endophytes isolated from M. oleifera leaves.

Figure 3
Figure 3

Phytotoxic activity of fungal metabolites from M. oleifera leaves.

Figure 4
Figure 4

Brine shrimp biolethality assay of leaves endophyts of M. oleifera.

Figure 5
Figure 5

Insecticidal activity of fungal metabolites isolated from M. oleifera leaves.

Figure 6
Figure 6

Larvicidal activity to control dengue fever by M. oleifera leaves endophytes.

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