pubmed.ncbi.nlm.nih.gov

Physiological Changes in the Levels of Anti-Cytokine Autoantibodies in Early Pregnancy Are Missing in Pregnant Women with Hashimoto's Thyroiditis - PubMed

  • ️Sun Jan 01 2023

Physiological Changes in the Levels of Anti-Cytokine Autoantibodies in Early Pregnancy Are Missing in Pregnant Women with Hashimoto's Thyroiditis

Szabina Erdő-Bonyár et al. J Immunol Res. 2023.

Abstract

T helper type 1 (Th1) and inflammatory cytokines play essential roles in early pregnancy and also in the pathogenesis of Hashimoto's thyroiditis (HT). Changes in the serum level of autoantibodies to cytokines, which may be able to modulate their availability and actions have been described in several autoimmune disorders. Yet, no data are available on anti-cytokine autoantibodies either during early pregnancy or in patients with HT. The aim of the study was to measure autoantibodies to inflammatory-, Th1- and Th22-cytokines in serum samples in healthy pregnancy (HP) and in pregnant women with HT (HTP). As pathological autoantibodies are hallmarks of HT, in addition we also measured anti-B-cell activator factor (BAFF) autoantibodies. The measurement was carried out with a Luminex multiplex assay and the Luminex MAGPIX Instrument, age-matched healthy women (HC) and women with HT (HT) were used as controls. In the first trimester of HP, anti-TNFα, anti-IL-8, and anti-IFNγ autoantibodies were significantly decreased, while autoantibodies to BAFF were significantly elevated compared to the HC. However, these alterations were not present in the HTP. Moreover, the levels of autoantibodies to IL-22 and TNFα were significantly increased in HTP compared to the HP. All differences in the levels of the investigated autoantibodies could be detected in the first trimester of pregnancies except for anti-IL-22 autoantibodies. According to our results we can conclude that alterations in the levels of autoantibodies to inflammatory and Th1 cytokines are physiological in the first trimester of pregnancy and their disturbance can be associated with autoimmune conditions such as HT.

Copyright © 2023 Szabina Erdő-Bonyár et al.

PubMed Disclaimer

Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1

Investigation of autoantibodies against two Th1-related cytokines in HC, HP, HT, and HTP. The serum levels of anti-IFNγ and anti-IL-22 autoantibodies were tested in all samples of HC (n = 10), HP (n = 23), HT (n = 13), HTP (n = 14) (a, b) and examined in the first and third trimester samples separately in HP (ntrim1 = 13, ntrim3 = 10) and HTP (ntrim1 = 8, ntrim3 = 6) (c, d). The boxes represent the interquartile ranges (IQR), the horizontal lines the medians and the whiskers the lowest and highest values.  ∗p < 0.05. The dashed lines show the trends, where p is between 0.05 and 1.

Figure 2
Figure 2

The difference in the serum level of autoantibodies against two pro-inflammatory cytokines. The levels of anti-TNFα and anti-IL-8 autoantibodies were measured in all samples of HC (n = 10), HP (n = 23), HT (n = 13), HTP (n = 14) (a, b) and in the first and third trimester samples separately in HP (ntrim1 = 13, ntrim3 = 10) and HTP (ntrim1 = 8, ntrim3 = 6) (c, d). The boxes represent the interquartile ranges (IQR), the horizontal lines the medians and the whiskers the lowest and highest values.  ∗p < 0.05. The dashed lines show the trends, where p is between 0.05 and 1.

Figure 3
Figure 3

Measurements of autoantibodies against BAFF in HC, HP, and HTP serum samples. Levels of anti-BAFF autoantibodies were investigated in all samples of HC (n = 10), HP (n = 23), HT (n = 13), HTP (n = 14) (a) and separately in the first and third trimester samples in HP (ntrim1 = 13, ntrim3 = 10) and HTP (ntrim1 = 8, ntrim3 = 6) (b). The boxes represent the interquartile ranges (IQR), the horizontal lines the medians and the whiskers the lowest and highest values.  ∗p < 0.05.

Similar articles

References

    1. Vincent T., Plawecki M., Goulabchand R., Guilpain P., Eliaou J. F. Emerging clinical phenotypes associated with anti-cytokine autoantibodies. Autoimmunity Reviews . 2015;14(6):528–535. doi: 10.1016/j.autrev.2015.01.015. - DOI - PubMed
    1. Cappellano G., Orilieri E., Woldetsadik A. D., et al. Anti-cytokine autoantibodies in autoimmune diseases. American Journal of Clinical and Experimental Immunology . 2012;1(2):136–146. - PMC - PubMed
    1. Jurisic V. Multiomic analysis of cytokines in immuno-oncology. Expert Review of Proteomics . 2020;17(9):663–674. doi: 10.1080/14789450.2020.1845654. - DOI - PubMed
    1. Jurisic V., Bogdanovic G., Kojic V., Jakimov D., Srdic T. Effect of TNF-α on Raji cells at different cellular levels estimated by various methods. Annals of Hematology . 2006;85(2):86–94. doi: 10.1007/s00277-005-0010-3. - DOI - PubMed
    1. Jurisic V., Terzic T., Colic S., Jurisic M. The concentration of TNF-α correlate with number of inflammatory cells and degree of vascularization in radicular cysts. Oral Diseases . 2008;14(7):600–605. doi: 10.1111/j.1601-0825.2007.01426.x. - DOI - PubMed

MeSH terms

Substances