DNA sequences of promoter regions for rRNA operons rrnE and rrnA in E. coli - PubMed

DNA sequences of promoter regions for rRNA operons rrnE and rrnA in E. coli

H A de Boer et al. Cell. 1979 May.

Abstract

The nucleotide sequences have been determined for the promoter regions of two ribosomal RNA operons, rrnA and rrnE, in E. coli. The sequences cover the two in vitro transcription start sites identified for each operon (Gilbert, der Boer and Nomura, 1979). The first two start sites are 283 and 291 bp preceding the mature 16S rRNA (m16S rNA) coding regions for rrnE and rrnA, respectively; the second start sites are 174 and 174 +/- 1 bp preceding the m16S rRNA coding regions for rrnE and rrnA, respectively. Each of these start sites has an identifiable "Pribnow box" sequence 6-7 bp upstream from the start site. The nucleotide sequences of the two operons have nearly complete homology from the m16S rRNA coding regions to positions 145 bp upstream from those regions, and at the regions surrounding the Pribnow boxes preceding the first start sites. The DNA sequences indicate that the RNAs transcribed from the first start sites of rrnE and rrnA are quite different in their first 150 nucleotides. These heterogeneous regions, however, precede the RNAse III cleavage sites (deduced previously by Young and Steitz, 1978), and the "precursor 16S rRNA" molecules are largely homogeneous. The nucleotide sequences of the promoter regions of the two rRNA operons are also compared with those or rrnD and rrnX, determined by Young and Steitz (1979), and some common features are discussed.

Similar articles

• Identification of initiation sites for the in vitro transcription of rRNA operons rrnE and rrnA in E. coli.

  Gilbert SF, de Boer HA, Nomura M. Gilbert SF, et al. Cell. 1979 May;17(1):211-24. doi: 10.1016/0092-8674(79)90309-x. Cell. 1979. PMID: 378406

• Tandem promoters direct E. coli ribosomal RNA synthesis.

  Young RA, Steitz JA. Young RA, et al. Cell. 1979 May;17(1):225-34. doi: 10.1016/0092-8674(79)90310-6. Cell. 1979. PMID: 110460

• Complementary sequences 1700 nucleotides apart form a ribonuclease III cleavage site in Escherichia coli ribosomal precursor RNA.

  Young RA, Steitz JA. Young RA, et al. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3593-7. doi: 10.1073/pnas.75.8.3593. Proc Natl Acad Sci U S A. 1978. PMID: 358189 Free PMC article.

• The seven E. coli ribosomal RNA operon upstream regulatory regions differ in structure and transcription factor binding efficiencies.

  Hillebrand A, Wurm R, Menzel A, Wagner R. Hillebrand A, et al. Biol Chem. 2005 Jun;386(6):523-34. doi: 10.1515/BC.2005.062. Biol Chem. 2005. PMID: 16006239

• DNA sequences of promoter regions for the str and spc ribosomal protein operons in E. coli.

  Post LE, Arfsten AE, Reusser F, Nomura M. Post LE, et al. Cell. 1978 Sep;15(1):215-29. doi: 10.1016/0092-8674(78)90096-x. Cell. 1978. PMID: 151587

Cited by

• In vivo translation of a region within the rrnB 16S rRNA gene of Escherichia coli.

  Berg KL, Squires CL, Squires C. Berg KL, et al. J Bacteriol. 1987 Apr;169(4):1691-701. doi: 10.1128/jb.169.4.1691-1701.1987. J Bacteriol. 1987. PMID: 2435709 Free PMC article.

• Value of a newly sequenced bacterial genome.

  Barbosa EG, Aburjaile FF, Ramos RT, Carneiro AR, Le Loir Y, Baumbach J, Miyoshi A, Silva A, Azevedo V. Barbosa EG, et al. World J Biol Chem. 2014 May 26;5(2):161-8. doi: 10.4331/wjbc.v5.i2.161. World J Biol Chem. 2014. PMID: 24921006 Free PMC article. Review.

• Initiation of Escherichia coli ribosomal RNA synthesis in vivo.

  Lund E, Dahlberg JE. Lund E, et al. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5480-4. doi: 10.1073/pnas.76.11.5480. Proc Natl Acad Sci U S A. 1979. PMID: 392503 Free PMC article.

• Promoter selectivity of E. coli RNA polymerase: analysis of the promoter system of convergently-transcribed dnaQ-rnh genes.

  Nomura T, Fujita N, Ishihama A. Nomura T, et al. Nucleic Acids Res. 1985 Nov 11;13(21):7647-61. doi: 10.1093/nar/13.21.7647. Nucleic Acids Res. 1985. PMID: 2999701 Free PMC article.

• Fusion of the promoter region of rRNA operon rrnB to lac Z gene.

  Glaser G, Kobi S, Oppenheim AB. Glaser G, et al. Nucleic Acids Res. 1980 Oct 10;8(19):4327-35. doi: 10.1093/nar/8.19.4327. Nucleic Acids Res. 1980. PMID: 6253913 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc