Evaluation and identification of powdery mildew-resistant genes in 137 wheat relatives - PubMed
- ️Mon Jan 01 2024
(A, B) Amplification patterns of specific markers CIT02g-18 for Pm6 (A) and Pm37-82 for Pm37 (B) in part of T. timopheevii Zhuk. M: pUC19/Msp I; Lane 1: Coker747 (A) and 66 (B); Lane 2: ChineseSpring; and Lanes 3–11: CWI 80427, CWI 80540, CWI 17007, CWI 17224, CWI 17259, CWI 17281, CWI 18175, CWI 18564, and CWI 18594. (C–F) Amplification patterns of specific markers MBH1 for Pm12 (C), STS-Pm24 for Pm24 (D), sfr43 for Pm8 (E), and Pm60-S1 for Pm60 (F) in part of T. monococcum L. M: pUC19/Msp I (C–D), DNA marker DL1200 (E–F); Lane 1: Yangmai 5 (C), Chiyacao (D), Disponent (E), and EK516 (F); Lane 2: ChineseSpring; and Lanes 3–17: CWI 96237, CWI 96263, CWI 96272, CWI 96275, CWI 17058, CWI 17154, CWI 18949, CWI 19498, CWI 19529, CWI 4393, CWI 4416, CWI 5110, CWI 93034, CWI 93289, and CWI 94083. (G–J): Amplification patterns of specific markers Pm2b-map-3 for Pm2 (G), Xsts24035 for Pm58 (H), Xdw12 for Pm68 (I), and JS717/718 for Pm4 (J) in part of T. aestivum subsp. spelta. M: pUC19/Msp I (G–J), DNA marker DL1200 (J); Lane 1: KM2939 (G), AY40 (H), TRI 1796 (I), and VPM1 (J); Lane 2: ChineseSpring; Lanes 3–17 (G): CWI 18468, CWI 14929, CWI 17063, CWI 17247, CWI 17324, CWI 17370, CWI 17677, CWI 17750, CWI 17879, CWI 17892, CWI 17942, CWI 17942, CWI 17954, CWI 17959, and CWI 17964; Lanes 3–17 (H): CWI 17370, CWI 18434, CWI 18439, CWI 18475, CWI 18476, CWI 44154, CWI 44215, CWI 44218, CWI 44409, CWI 80462, CWI 80503, CWI 92915, CWI 96275, CWI 96277, and CWI 96279; Lanes 3–17 (I): CWI 18434, CWI 18439, CWI 14929, CWI 17063, CWI 17247, CWI 17324, CWI 17370, CWI 17677, CWI 17750, CWI 17879, CWI 17892, CWI 17942, CWI 17942, CWI 17954, and CWI 17959; and Lanes 3–17 (J): CWI 18477, CWI 78977, CWI 78976, CWI 80531, CWI 14929, CWI 17063, CWI 17247, CWI 17324, CWI 17370, CWI 17677, CWI 17750, CWI 17879, CWI 17892, CWI 17942, and CWI 17942. Red arrows indicate specific bands.