Saccharomyces cerevisiae CYC1 mRNA 5'-end positioning: analysis by in vitro mutagenesis, using synthetic duplexes with random mismatch base pairs - PubMed

Saccharomyces cerevisiae CYC1 mRNA 5'-end positioning: analysis by in vitro mutagenesis, using synthetic duplexes with random mismatch base pairs

J B McNeil et al. Mol Cell Biol. 1985 Dec.

Abstract

Expression of the Saccharomyces cerevisiae CYC1 gene produces mRNA with more than 20 different 5' ends. A derivative of the CYC1 gene (CYC1-157) was constructed with a deletion of a portion of the CYC1 5'-noncoding region, which includes the sites at which many of the CYC1 mRNAs 5' ends map. A 54-mer double-stranded oligonucleotide homologous with the deleted sequence of CYC1-157 and which included a low level of random base pair mismatches (an average of two mismatches per duplex) was used to construct mutants of the CYC1 gene and examine the role of the DNA sequence at and immediately adjacent to the mRNA 5' ends in specifying their locations. The effect of these mutations on the site selection of mRNA 5' ends was examined by primer extension. Results indicate that there is a strong preference for 5' ends which align with an A residue (T in the template DNA strand) preceded by a short tract of pyrimidine residues.

Similar articles

• Transcription initiation of the Saccharomyces cerevisiae iso-1-cytochrome c gene. Multiple, independent T-A-T-A sequences.

  McNeil JB, Smith M. McNeil JB, et al. J Mol Biol. 1986 Feb 5;187(3):363-78. doi: 10.1016/0022-2836(86)90439-0. J Mol Biol. 1986. PMID: 3009831

• Signals that produce 3' termini in CYC1 mRNA of the yeast Saccharomyces cerevisiae.

  Russo P, Li WZ, Guo Z, Sherman F. Russo P, et al. Mol Cell Biol. 1993 Dec;13(12):7836-49. doi: 10.1128/mcb.13.12.7836-7849.1993. Mol Cell Biol. 1993. PMID: 8246998 Free PMC article.

• Distinct cis-acting signals enhance 3' endpoint formation of CYC1 mRNA in the yeast Saccharomyces cerevisiae.

  Russo P, Li WZ, Hampsey DM, Zaret KS, Sherman F. Russo P, et al. EMBO J. 1991 Mar;10(3):563-71. doi: 10.1002/j.1460-2075.1991.tb07983.x. EMBO J. 1991. PMID: 1848175 Free PMC article.

• Mutationally altered 3' ends of yeast CYC1 mRNA affect transcript stability and translational efficiency.

  Zaret KS, Sherman F. Zaret KS, et al. J Mol Biol. 1984 Jul 25;177(1):107-35. doi: 10.1016/0022-2836(84)90060-3. J Mol Biol. 1984. PMID: 6086937

• The importance of mutation, then and now: studies with yeast cytochrome c.

  Sherman F. Sherman F. Mutat Res. 2005 Jan;589(1):1-16. doi: 10.1016/j.mrrev.2004.07.001. Mutat Res. 2005. PMID: 15652223 Review.

Cited by

• Properties of promoters cloned randomly from the Saccharomyces cerevisiae genome.

  Santangelo GM, Tornow J, McLaughlin CS, Moldave K. Santangelo GM, et al. Mol Cell Biol. 1988 Oct;8(10):4217-24. doi: 10.1128/mcb.8.10.4217-4224.1988. Mol Cell Biol. 1988. PMID: 2847031 Free PMC article.

• Efficient expression of the Saccharomyces cerevisiae PGK gene depends on an upstream activation sequence but does not require TATA sequences.

  Ogden JE, Stanway C, Kim S, Mellor J, Kingsman AJ, Kingsman SM. Ogden JE, et al. Mol Cell Biol. 1986 Dec;6(12):4335-43. doi: 10.1128/mcb.6.12.4335-4343.1986. Mol Cell Biol. 1986. PMID: 3540610 Free PMC article.

• Construction of interleukin-1 alpha mutants using unequal contamination of synthetic oligonucleotides.

  Poindexter K, Jerzy R, Gayle RB 3rd. Poindexter K, et al. Nucleic Acids Res. 1991 Apr 25;19(8):1899-904. doi: 10.1093/nar/19.8.1899. Nucleic Acids Res. 1991. PMID: 1840657 Free PMC article.

• A transcriptional barrier to expression of cloned toxin genes of the linear plasmid k1 of Kluyveromyces lactis: evidence that native k1 has novel promoters.

  Romanos MA, Boyd A. Romanos MA, et al. Nucleic Acids Res. 1988 Aug 11;16(15):7333-50. doi: 10.1093/nar/16.15.7333. Nucleic Acids Res. 1988. PMID: 3412888 Free PMC article.

• Dominant-negative mutant phenotypes and the regulation of translation elongation factor 2 levels in yeast.

  Ortiz PA, Kinzy TG. Ortiz PA, et al. Nucleic Acids Res. 2005 Oct 6;33(18):5740-8. doi: 10.1093/nar/gki882. Print 2005. Nucleic Acids Res. 2005. PMID: 16214807 Free PMC article.

References

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc
  • Saccharomyces Genome Database

• Miscellaneous

  • NCI CPTAC Assay Portal