Mapping adenines, guanines, and pyrimidines in RNA - PubMed

Mapping adenines, guanines, and pyrimidines in RNA

H Donis-Keller et al. Nucleic Acids Res. 1977 Aug.

Free PMC article

Abstract

The positions of adenines, guanines, and pyrimidines can be determined by partial nuclease digestion of a terminally labeles RNA molecule. In urea, at elevated temperatures, RNase T1 generates a pattern reflecting cleavage at guanines while RNase U2 cleaves only at adenine. A limited alkaline hydrolysis provides a continuum of fragments derived from breaks at every phosphodiester bond. The reaction products are electrophoretically fractionated by size in adjacent lanes of a polyacrylamide gel. An autoradiograph of the gel displays the sequence up to 100 nucleotides from the end of the molecule, although uracil cannot as yet be distinguished from cytosine. These techniques form the basis of an RNA sequencing method and are demonstrated on yeast 5.8S ribosomal RNA.

Similar articles

• [Primary structure of valine transfer RNA I of baker's yeast. Nucleotide content and oligonucleotides in ribonuclease hydrolysates].

  Venkstern TV, Li L, Krutilina AI, Mirzabekov AD, Aksel'rod VD, Baev AA. Venkstern TV, et al. Dokl Akad Nauk SSSR. 1967 Mar 11;173(2):459-62. Dokl Akad Nauk SSSR. 1967. PMID: 5623092 Russian. No abstract available.

• Yeast tRNA Leu UAG. Purification, properties and determination of the nucleotide sequence by radioactive derivative methods.

  Randerath E, Gupta RC, Chia LL, Chang SH, Randerath K. Randerath E, et al. Eur J Biochem. 1979 Jan 2;93(1):79-94. doi: 10.1111/j.1432-1033.1979.tb12797.x. Eur J Biochem. 1979. PMID: 374075

• A new method for sequencing DNA.

  Maxam AM, Gilbert W. Maxam AM, et al. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4. doi: 10.1073/pnas.74.2.560. Proc Natl Acad Sci U S A. 1977. PMID: 265521 Free PMC article.

• [Gas chromatography of nucleic acid components].

  Hashizume T, Sasaki Y. Hashizume T, et al. Tanpakushitsu Kakusan Koso. 1968 Jul;13(8):735-42. Tanpakushitsu Kakusan Koso. 1968. PMID: 4882393 Review. Japanese. No abstract available.

• Crystallographic study of mechanism of ribonuclease T1-catalysed specific RNA hydrolysis.

  Heinemann U, Saenger W. Heinemann U, et al. J Biomol Struct Dyn. 1983 Oct;1(2):523-38. doi: 10.1080/07391102.1983.10507459. J Biomol Struct Dyn. 1983. PMID: 6086061 Review.

Cited by

• Third-generation sequencing techniques and applications to drug discovery.

  Ozsolak F. Ozsolak F. Expert Opin Drug Discov. 2012 Mar;7(3):231-43. doi: 10.1517/17460441.2012.660145. Epub 2012 Feb 2. Expert Opin Drug Discov. 2012. PMID: 22468954 Free PMC article. Review.

• Challenges and emerging trends in liquid chromatography-based analyses of mRNA pharmaceuticals.

  Fekete S, Doneanu C, Addepalli B, Gaye M, Nguyen J, Alden B, Birdsall R, Han D, Isaac G, Lauber M. Fekete S, et al. J Pharm Biomed Anal. 2023 Feb 5;224:115174. doi: 10.1016/j.jpba.2022.115174. Epub 2022 Nov 21. J Pharm Biomed Anal. 2023. PMID: 36446261 Free PMC article. Review.

• Location of transcriptional control signals and transfer RNA sequences in Torulopsis glabrata mitochondrial DNA.

  Clark-Walker GD, McArthur CR, Sriprakash KS. Clark-Walker GD, et al. EMBO J. 1985 Feb;4(2):465-73. doi: 10.1002/j.1460-2075.1985.tb03652.x. EMBO J. 1985. PMID: 4040462 Free PMC article.

• Chemical synthesis of the 24 RNA fragments corresponding to hop stunt viroid.

  Tanimura H, Maeda M, Fukazawa T, Sekine M, Hata T. Tanimura H, et al. Nucleic Acids Res. 1989 Oct 25;17(20):8135-47. doi: 10.1093/nar/17.20.8135. Nucleic Acids Res. 1989. PMID: 2813059 Free PMC article.

• Analysis of transcripts of the major cluster of tRNA genes in the mitochondrial genome of S. cerevisiae.

  Francisci S, Palleschi C, Ragnini A, Frontali L. Francisci S, et al. Nucleic Acids Res. 1987 Aug 25;15(16):6387-403. doi: 10.1093/nar/15.16.6387. Nucleic Acids Res. 1987. PMID: 3306600 Free PMC article.

References

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • PubMed Central
  • Silverchair Information Systems

• Other Literature Sources

  • The Lens - Patent Citations Database