Sodium butyrate stimulates somatostatin production by cultured cells - PubMed

Sodium butyrate stimulates somatostatin production by cultured cells

R Green et al. Endocrinology. 1984 Jun.

Abstract

Expression of the neuropeptide SRIF can be enhanced by sodium butyrate, a known modulator of gene transcription. Two different cell lines, RIN cells (derived from a rat pancreatic islet tumor) as well as HeLa cells, showed basal secretion of SRIF immunoreactive material that was induced between 5- and 15-fold by sodium butyrate. The induction was dose and time dependent, fully reversible, and specific for sodium butyrate. Analysis of the induced mRNAs by Northern blot hybridization using a SRIF specific cDNA probe revealed a 12-fold increase in the level of SRIF mRNA caused by butyrate treatment. These results suggest that sodium butyrate may be a useful tool for studying the regulation of SRIF gene expression.

Similar articles

• Clonal analysis of insulin and somatostatin secretion and L-dopa decarboxylase expression by a rat islet cell tumor.

  Oie HK, Gazdar AF, Minna JD, Weir GC, Baylin SB. Oie HK, et al. Endocrinology. 1983 Mar;112(3):1070-5. doi: 10.1210/endo-112-3-1070. Endocrinology. 1983. PMID: 6129963

• Sodium butyrate induces neuroendocrine cytodifferentiation in the insulinoma cell line RINm5F.

  Swarovsky B, Eissele R, Eisenacher M, Trautmann ME, Arnold R. Swarovsky B, et al. Pancreas. 1994 Jul;9(4):460-8. doi: 10.1097/00006676-199407000-00008. Pancreas. 1994. PMID: 7937695

• Effect of sodium butyrate on glucose transport and glucose-phosphorylating enzymes in RIN-m5F cells.

  Fernandez-Mejia C, Davidson MB. Fernandez-Mejia C, et al. Pancreas. 1993 Sep;8(5):589-96. doi: 10.1097/00006676-199309000-00010. Pancreas. 1993. PMID: 8302795

• Defective regulation of insulin secretion in diabetes and insulinoma.

  Flatt PR. Flatt PR. Biochem Soc Trans. 1990 Feb;18(1):124-7. doi: 10.1042/bst0180124. Biochem Soc Trans. 1990. PMID: 2158914 Review. No abstract available.

Cited by

• Unusual c-fos induction upon chromaffin PC12 differentiation by sodium butyrate: loss of fos autoregulatory function.

  Naranjo JR, Mellström B, Auwerx J, Mollinedo F, Sassone-Corsi P. Naranjo JR, et al. Nucleic Acids Res. 1990 Jun 25;18(12):3605-10. doi: 10.1093/nar/18.12.3605. Nucleic Acids Res. 1990. PMID: 2114014 Free PMC article.

• Opposite effects of sodium butyrate on CCK mRNA and CCK peptide levels in RIN cells.

  Roche C, Cordier-Bussat M, Ratineau C, Bernard C, Philippe J, Cuber JC. Roche C, et al. Endocrine. 1996 Dec;5(3):331-4. doi: 10.1007/BF02739067. Endocrine. 1996. PMID: 21153085

• Transcriptional regulation of genes encoding insulin, glucagon, and angiotensinogen by sodium butyrate in a rat islet cell line.

  Philippe J, Drucker DJ, Chick WL, Habener JF. Philippe J, et al. Mol Cell Biol. 1987 Jan;7(1):560-3. doi: 10.1128/mcb.7.1.560-563.1987. Mol Cell Biol. 1987. PMID: 3550424 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Silverchair Information Systems

• Medical

  • MedlinePlus Health Information