Protein synthesis by intact Coxiella burnetii cells - PubMed
Protein synthesis by intact Coxiella burnetii cells
R L Zuerner et al. J Bacteriol. 1983 Oct.
Abstract
Coxiella burnetii was isolated from persistently infected fibroblast host cells by a rapid mechanical lysis technique. Macromolecular synthesis was initiated in these otherwise dormant cells by incubation at pH 4.5. The synthesis of protein proceeded for as long as 24 h. Initiation of protein synthesis in C. burnetii was dependent upon RNA synthesis. Approximately 24 species of polypeptides were synthesized, and some of these appeared to be major synthetic products. Increases in protein biomass of 15 to 30% were calculated to occur during incubation. Inhibition of DNA synthesis affected protein synthesis after 12 h of incubation. The results suggest that although these parasitic bacteria did not grow in the axenic media devised, significant biosynthetic processes occurred.
Similar articles
-
Isolated Coxiella burnetii synthesizes DNA during acid activation in the absence of host cells.
Chen SY, Vodkin M, Thompson HA, Williams JC. Chen SY, et al. J Gen Microbiol. 1990 Jan;136(1):89-96. doi: 10.1099/00221287-136-1-89. J Gen Microbiol. 1990. PMID: 2351956
-
Christian RG, Paretsky D. Christian RG, et al. J Bacteriol. 1977 Dec;132(3):841-6. doi: 10.1128/jb.132.3.841-846.1977. J Bacteriol. 1977. PMID: 200603 Free PMC article.
-
Secretion of proteins by Coxiella burnetii.
Redd T, Thompson HA. Redd T, et al. Microbiology (Reading). 1995 Feb;141 ( Pt 2):363-9. doi: 10.1099/13500872-141-2-363. Microbiology (Reading). 1995. PMID: 7704266
-
Effects of temperature upon the cell-free translation system from Coxiella burnetii.
Donahue JP, Thompson HA. Donahue JP, et al. Can J Microbiol. 1987 Jul;33(7):642-6. doi: 10.1139/m87-112. Can J Microbiol. 1987. PMID: 3664368
-
Synthesis of serine by Coxiella burnetii.
MYERS WF, PARETSKY D. MYERS WF, et al. J Bacteriol. 1961 Nov;82(5):761-3. doi: 10.1128/jb.82.5.761-763.1961. J Bacteriol. 1961. PMID: 14477717 Free PMC article.
Cited by
-
Maurin M, Raoult D. Maurin M, et al. Clin Microbiol Rev. 1999 Oct;12(4):518-53. doi: 10.1128/CMR.12.4.518. Clin Microbiol Rev. 1999. PMID: 10515901 Free PMC article. Review.
-
Maurin M, Benoliel AM, Bongrand P, Raoult D. Maurin M, et al. Infect Immun. 1992 Dec;60(12):5013-6. doi: 10.1128/iai.60.12.5013-5016.1992. Infect Immun. 1992. PMID: 1452331 Free PMC article.
-
Cloning and characterization of an autonomous replication sequence from Coxiella burnetii.
Suhan M, Chen SY, Thompson HA, Hoover TA, Hill A, Williams JC. Suhan M, et al. J Bacteriol. 1994 Sep;176(17):5233-43. doi: 10.1128/jb.176.17.5233-5243.1994. J Bacteriol. 1994. PMID: 8071197 Free PMC article.
-
Interaction of chlamydiae and host cells in vitro.
Moulder JW. Moulder JW. Microbiol Rev. 1991 Mar;55(1):143-90. doi: 10.1128/mr.55.1.143-190.1991. Microbiol Rev. 1991. PMID: 2030670 Free PMC article. Review.
-
Sustained axenic metabolic activity by the obligate intracellular bacterium Coxiella burnetii.
Omsland A, Cockrell DC, Fischer ER, Heinzen RA. Omsland A, et al. J Bacteriol. 2008 May;190(9):3203-12. doi: 10.1128/JB.01911-07. Epub 2008 Feb 29. J Bacteriol. 2008. PMID: 18310349 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources