Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs - PubMed

• PMID: 6694911
• PMCID: PMC318541
• DOI: 10.1093/nar/12.2.857

Free PMC article

Comparative Study

Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs

M Kozak. Nucleic Acids Res. 1984.

Free PMC article

Abstract

5-Noncoding sequences have been tabulated for 211 messenger RNAs from higher eukaryotic cells. The 5'-proximal AUG triplet serves as the initiator codon in 95% of the mRNAs examined. The most conspicuous conserved feature is the presence of a purine (most often A) three nucleotides upstream from the AUG initiator codon; only 6 of the mRNAs in the survey have a pyrimidine in that position. There is a predominance of C in positions -1, -2, -4 and -5, just upstream from the initiator codon. The sequence CCAGCCAUG (G) thus emerges as a consensus sequence for eukaryotic initiation sites. The extent to which the ribosome binding site in a given mRNA matches the -1 to -5 consensus sequence varies: more than half of the mRNAs in the tabulation have 3 or 4 nucleotides in common with the CCACC consensus, but only ten mRNAs conform perfectly.

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