The role of mitochondria in growth plate calcification as demonstrated in a rachitic model - PubMed

• PMID: 681381

The role of mitochondria in growth plate calcification as demonstrated in a rachitic model

C T Brighton et al. J Bone Joint Surg Am. 1978 Jul.

Abstract

In a phosphate-vitamin D-deficiency rachitic model in rats, potassium pyroantimonate was employed as a histochemical stain for calcium at the ultrastructural level in the costochondral growth plates. In the control plates, there was a shift of calcium from an intracellular, mainly mitochondrial location in the top part of the zone of hypertrophic cells to an extracellular, mainly matrix-vesicle location in the bottom part of the zone of hypertrophic cells. In the rachitic plates, mitochondria and cell membranes throughout the bottom of the hypertrophic zone remained heavily loaded with calcium. After treatment with phosphate was started, the mitochondria and cell membranes at the bottom of the hypertrophic zone rapidly lost calcium and matrix calcification began. Thus, in the normal growth plate, matrix calcification begins at the level in the plate where mitochondria lose calcium; in rickets, the matrix does not calcify and mitochondria do not lose calcium; and in healing rickets, calcification begins in the matrix at the bottom of the hypertrophic zone as the mitochondria at that level lose calcium. These findings support the hypothesis that mitochondria play an important role in matrix calcification.

Similar articles

• Histochemical localization of calcium in growth plate mitochondria and matrix vesicles.

  Brighton CT, Hunt RM. Brighton CT, et al. Fed Proc. 1976 Feb;35(2):143-7. Fed Proc. 1976. PMID: 1248647

• Calcification of rachitic rat cartilage in vitro by extracellular matrix vesicles.

  Anderson HC, Cecil R, Sajdera SW. Anderson HC, et al. Am J Pathol. 1975 May;79(2):237-54. Am J Pathol. 1975. PMID: 1146961 Free PMC article.

• Calcification of rachitic cartilage to study matrix vesicle function.

  Anderson HC, Sajdera SW. Anderson HC, et al. Fed Proc. 1976 Feb;35(2):148-53. Fed Proc. 1976. PMID: 1248648

• Calcification mechanisms.

  Howell DS. Howell DS. Isr J Med Sci. 1976 Feb;12(2):91-7. Isr J Med Sci. 1976. PMID: 770394 Review. No abstract available.

• Comparison of normal and rachitic rat matrix vesicles.

  Anderson HC, Stechschulte DJ Jr, Hsu HH, Morris DC. Anderson HC, et al. Bone Miner. 1992 May;17(2):119-22. doi: 10.1016/0169-6009(92)90721-o. Bone Miner. 1992. PMID: 1611295 Review.

Cited by

• Disrupted mitochondrial function in the Opa3L122P mouse model for Costeff Syndrome impairs skeletal integrity.

  Navein AE, Cooke EJ, Davies JR, Smith TG, Wells LH, Ohazama A, Healy C, Sharpe PT, Evans SL, Evans BA, Votruba M, Wells T. Navein AE, et al. Hum Mol Genet. 2016 Jun 15;25(12):2404-2416. doi: 10.1093/hmg/ddw107. Epub 2016 Apr 22. Hum Mol Genet. 2016. PMID: 27106103 Free PMC article.

• Effect of magnesium on lipid-induced calcification: an in vitro model for bone mineralization.

  Boskey AL, Posner AS. Boskey AL, et al. Calcif Tissue Int. 1980;32(2):139-43. doi: 10.1007/BF02408533. Calcif Tissue Int. 1980. PMID: 6773631

• Healing of rickets with phosphate supplementation in the hypophosphatemic male mouse.

  Marie PJ, Travers R, Glorieux FH. Marie PJ, et al. J Clin Invest. 1981 Mar;67(3):911-4. doi: 10.1172/jci110110. J Clin Invest. 1981. PMID: 6259210 Free PMC article.

• Active extrusion of Ca2+ from epiphysial chondrocytes of normal and rachitic chickens.

  Zanetti M, Camerotto R, Romeo D, De Bernard B. Zanetti M, et al. Biochem J. 1982 Feb 15;202(2):303-7. doi: 10.1042/bj2020303. Biochem J. 1982. PMID: 6807290 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Ovid Technologies, Inc.
  • Wolters Kluwer