Synthesis and assembly of hepatitis B virus surface antigen particles in yeast - PubMed

• PMID: 7045698
• DOI: 10.1038/298347a0

Synthesis and assembly of hepatitis B virus surface antigen particles in yeast

P Valenzuela et al. Nature. 1982.

Abstract

The surface antigens of hepatitis B virus (HBsAg) has been synthesized in the yeast Saccharomyces cerevisiae by using an expression vector that employs the 5'-flanking region of yeast alcohol dehydrogenase I as a promotor to transcribe surface antigen coding sequences. The protein synthesized in yeast is assembled into particles having properties similar to the 22-nm particles secreted by human cells.

Similar articles

• Expression of hepatitis B surface antigen gene in yeast.

  Miyanohara A, Toh-e A, Nozaki C, Hamada F, Ohtomo N, Matsubara K. Miyanohara A, et al. Proc Natl Acad Sci U S A. 1983 Jan;80(1):1-5. doi: 10.1073/pnas.80.1.1. Proc Natl Acad Sci U S A. 1983. PMID: 6337369 Free PMC article.

• [Expression of the HBsAg gene of the hepatitis B virus under the control of the promoter region of gene PH05 in yeast cells].

  Granovskiĭ NN, Zhdanov VM, Smirnov MN. Granovskiĭ NN, et al. Vopr Virusol. 1985 Sep-Oct;30(5):558-61. Vopr Virusol. 1985. PMID: 3907141 Russian.

• Characteristics of hepatitis B surface antigen produced in yeast.

  Choo KB, Wu SM, Lee HH, Lo SC. Choo KB, et al. Biochem Biophys Res Commun. 1985 Aug 30;131(1):160-6. doi: 10.1016/0006-291x(85)91784-x. Biochem Biophys Res Commun. 1985. PMID: 3899110

• [Structure and expression of human hepatitis B virus surface antigen].

  Kozlovskaia TM, Pumpen PP. Kozlovskaia TM, et al. Mol Biol (Mosk). 1986 Jul-Aug;20(4):884-901. Mol Biol (Mosk). 1986. PMID: 2429171 Review. Russian.

• DNA-mediated immunization to the hepatitis B surface antigen. Activation and entrainment of the immune response.

  Whalen RG, Leclerc C, Dériaud E, Schirmbeck R, Reimann J, Davis HL. Whalen RG, et al. Ann N Y Acad Sci. 1995 Nov 27;772:64-76. doi: 10.1111/j.1749-6632.1995.tb44732.x. Ann N Y Acad Sci. 1995. PMID: 8546414 Review.

Cited by

• Harnessing DNA-induced immune responses for improving cancer vaccines.

  Herrada AA, Rojas-Colonelli N, González-Figueroa P, Roco J, Oyarce C, Ligtenberg MA, Lladser A. Herrada AA, et al. Hum Vaccin Immunother. 2012 Nov 1;8(11):1682-93. doi: 10.4161/hv.22345. Epub 2012 Oct 30. Hum Vaccin Immunother. 2012. PMID: 23111166 Free PMC article. Review.

• Nature and display of hepatitis B virus envelope proteins and the humoral immune response.

  Alberti A, Gerlich WH, Heermann KH, Pontisso P. Alberti A, et al. Springer Semin Immunopathol. 1990;12(1):5-23. doi: 10.1007/BF00192678. Springer Semin Immunopathol. 1990. PMID: 1695029 Review. No abstract available.

• Viral and bacterial vectors of immunogenes.

  Cavanagh D. Cavanagh D. Vaccine. 1985 Mar;3(1):45-8. doi: 10.1016/0264-410x(85)90011-8. Vaccine. 1985. PMID: 4002835 Free PMC article.

• Papillomavirus L1 major capsid protein self-assembles into virus-like particles that are highly immunogenic.

  Kirnbauer R, Booy F, Cheng N, Lowy DR, Schiller JT. Kirnbauer R, et al. Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):12180-4. doi: 10.1073/pnas.89.24.12180. Proc Natl Acad Sci U S A. 1992. PMID: 1334560 Free PMC article.

• Impact of fungal molecular genetics on biotechnology.

  Strijkert PJ. Strijkert PJ. Antonie Van Leeuwenhoek. 1987;53(5):357-61. doi: 10.1007/BF00400561. Antonie Van Leeuwenhoek. 1987. PMID: 3318692 Review.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Nature Publishing Group

• Other Literature Sources

  • The Lens - Patent Citations Database