Assembly units of clathrin coats - PubMed

• PMID: 7464911
• DOI: 10.1038/289420a0

Assembly units of clathrin coats

E Ungewickell et al. Nature. 1981.

Abstract

Clathrin, a polypeptide of molecular weight (MW) 180,000, is the main constituent of the polygonal network that forms the coat of coated pits and vesicles; these vesicles play a part in intracellular transport between membranous organelles. This function involves specific recognition of target membranes as well as fusion and fission events that must be coordinated with the assembly, partial disassembly or reorganization of the clathrin coats. To understand these interactions on a molecular level, information about the structure of clathrin and the interactions of clathrin with itself and other proteins is required. Here we show that purified clathrin coats dissociate reversibly into triskelions, structures composed of three usually bent, rather flexible legs irradiated from a centre. We have determined the molecular weight of these triskelions and conclude that they contain trimers of clathrin together with about three light molecular weight polypeptide chains.

Similar articles

• Clathrin assembly proteins: affinity purification and a model for coat assembly.

  Keen JH. Keen JH. J Cell Biol. 1987 Nov;105(5):1989-98. doi: 10.1083/jcb.105.5.1989. J Cell Biol. 1987. PMID: 2890644 Free PMC article.

• Assembly polypeptides from coated vesicles mediate reassembly of unique clathrin coats.

  Zaremba S, Keen JH. Zaremba S, et al. J Cell Biol. 1983 Nov;97(5 Pt 1):1339-47. doi: 10.1083/jcb.97.5.1339. J Cell Biol. 1983. PMID: 6138359 Free PMC article.

• The association of clathrin fragments with coated vesicle membranes.

  Hanspal M, Luna E, Branton D. Hanspal M, et al. J Biol Chem. 1984 Sep 10;259(17):11075-82. J Biol Chem. 1984. PMID: 6147350

• Clathrin: anatomy of a coat protein.

  Smith CJ, Pearse BM. Smith CJ, et al. Trends Cell Biol. 1999 Sep;9(9):335-8. doi: 10.1016/s0962-8924(99)01631-1. Trends Cell Biol. 1999. PMID: 10461185 Review.

• Life of a clathrin coat: insights from clathrin and AP structures.

  Edeling MA, Smith C, Owen D. Edeling MA, et al. Nat Rev Mol Cell Biol. 2006 Jan;7(1):32-44. doi: 10.1038/nrm1786. Nat Rev Mol Cell Biol. 2006. PMID: 16493411 Review.

Cited by

• Coat proteins isolated from clathrin coated vesicles can assemble into coated pits.

  Mahaffey DT, Moore MS, Brodsky FM, Anderson RG. Mahaffey DT, et al. J Cell Biol. 1989 May;108(5):1615-24. doi: 10.1083/jcb.108.5.1615. J Cell Biol. 1989. PMID: 2565904 Free PMC article.

• Clathrin coats partially preassemble and subsequently bend during endocytosis.

  Mund M, Tschanz A, Wu YL, Frey F, Mehl JL, Kaksonen M, Avinoam O, Schwarz US, Ries J. Mund M, et al. J Cell Biol. 2023 Mar 6;222(3):e202206038. doi: 10.1083/jcb.202206038. Epub 2023 Feb 3. J Cell Biol. 2023. PMID: 36734980 Free PMC article.

• Clathrin assembly involves a light chain-binding region.

  Blank GS, Brodsky FM. Blank GS, et al. J Cell Biol. 1987 Nov;105(5):2011-9. doi: 10.1083/jcb.105.5.2011. J Cell Biol. 1987. PMID: 2445759 Free PMC article.

• Tunable circular dichroism through absorption in coupled optical modes of twisted triskelia nanostructures.

  Rodríguez-Álvarez J, García-Martín A, Fraile Rodríguez A, Batlle X, Labarta A. Rodríguez-Álvarez J, et al. Sci Rep. 2022 Jan 7;12(1):26. doi: 10.1038/s41598-021-03908-2. Sci Rep. 2022. PMID: 34996969 Free PMC article.

• A unique role for clathrin light chain A in cell spreading and migration.

  Tsygankova OM, Keen JH. Tsygankova OM, et al. J Cell Sci. 2019 May 15;132(10):jcs224030. doi: 10.1242/jcs.224030. J Cell Sci. 2019. PMID: 30975920 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Nature Publishing Group

• Other Literature Sources

  • The Lens - Patent Citations Database