Rolling replication of short DNA circles - PubMed

Rolling replication of short DNA circles

A Fire et al. Proc Natl Acad Sci U S A. 1995.

Abstract

Natural genes and proteins often contain tandemly repeated sequence motifs that dramatically increase physiological specificity and activity. Given the selective value of such repeats, it is likely that several different mechanisms have been responsible for their generation. One mechanism that has been shown to generate relatively long tandem repeats (in the kilobase range) is rolling circle replication. In this communication, we demonstrate that rolling circle synthesis in a simple enzymatic system can produce tandem repeats of monomers as short as 34 bp. In addition to suggesting possible origins for natural tandem repeats, these observations provide a facile means for constructing libraries of repeated motifs for use in "in vitro evolution" experiments designed to select molecules with defined biological or chemical properties.

Similar articles

• Circle-to-circle amplification for precise and sensitive DNA analysis.

  Dahl F, Banér J, Gullberg M, Mendel-Hartvig M, Landegren U, Nilsson M. Dahl F, et al. Proc Natl Acad Sci U S A. 2004 Mar 30;101(13):4548-53. doi: 10.1073/pnas.0400834101. Epub 2004 Mar 15. Proc Natl Acad Sci U S A. 2004. PMID: 15070755 Free PMC article.

• Design of DNA rolling-circle templates with controlled fork topology to study mechanisms of DNA replication.

  Monachino E, Ghodke H, Spinks RR, Hoatson BS, Jergic S, Xu ZQ, Dixon NE, van Oijen AM. Monachino E, et al. Anal Biochem. 2018 Sep 15;557:42-45. doi: 10.1016/j.ab.2018.07.008. Epub 2018 Jul 18. Anal Biochem. 2018. PMID: 30016625

• Extrachromosomal circular DNA in eukaryotes: possible involvement in the plasticity of tandem repeats.

  Cohen S, Segal D. Cohen S, et al. Cytogenet Genome Res. 2009;124(3-4):327-38. doi: 10.1159/000218136. Epub 2009 Jun 25. Cytogenet Genome Res. 2009. PMID: 19556784 Review.

• Rolling-circle amplification in DNA diagnostics: the power of simplicity.

  Demidov VV. Demidov VV. Expert Rev Mol Diagn. 2002 Nov;2(6):542-8. doi: 10.1586/14737159.2.6.542. Expert Rev Mol Diagn. 2002. PMID: 12465451 Review.

Cited by

• A Loop-Mediated Isothermal Amplification Assay for the Rapid Detection of Didymella segeticola Causing Tea Leaf Spot.

  Tu Y, Wang Y, Jiang H, Ren H, Wang X, Lv W. Tu Y, et al. J Fungi (Basel). 2024 Jul 3;10(7):467. doi: 10.3390/jof10070467. J Fungi (Basel). 2024. PMID: 39057352 Free PMC article.

• Electrical and electrochemical monitoring of nucleic Acid amplification.

  Goda T, Tabata M, Miyahara Y. Goda T, et al. Front Bioeng Biotechnol. 2015 Mar 5;3:29. doi: 10.3389/fbioe.2015.00029. eCollection 2015. Front Bioeng Biotechnol. 2015. PMID: 25798440 Free PMC article. Review.

• Strategies for signal amplification in nucleic acid detection.

  Andras SC, Power JB, Cocking EC, Davey MR. Andras SC, et al. Mol Biotechnol. 2001 Sep;19(1):29-44. doi: 10.1385/MB:19:1:029. Mol Biotechnol. 2001. PMID: 11697219 Review.

• A new enzymatic route for production of long 5'-phosphorylated oligonucleotides using suicide cassettes and rolling circle DNA synthesis.

  Lohmann JS, Stougaard M, Koch J. Lohmann JS, et al. BMC Biotechnol. 2007 Aug 16;7:49. doi: 10.1186/1472-6750-7-49. BMC Biotechnol. 2007. PMID: 17705815 Free PMC article.

• The Potential Use of Isothermal Amplification Assays for In-Field Diagnostics of Plant Pathogens.

  Ivanov AV, Safenkova IV, Zherdev AV, Dzantiev BB. Ivanov AV, et al. Plants (Basel). 2021 Nov 10;10(11):2424. doi: 10.3390/plants10112424. Plants (Basel). 2021. PMID: 34834787 Free PMC article. Review.

References

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations Database