Chloroplast and cytosolic triosephosphate isomerases from spinach: purification, microsequencing and cDNA cloning of the chloroplast enzyme - PubMed

Affiliations

• PMID: 7858230
• DOI: 10.1007/BF00019506

Comparative Study

Chloroplast and cytosolic triosephosphate isomerases from spinach: purification, microsequencing and cDNA cloning of the chloroplast enzyme

K Henze et al. Plant Mol Biol. 1994 Dec.

Abstract

Chloroplast and cytosolic triosephosphate isomerases from spinach were separated and purified to homogeneity. Both enzymes were partially sequenced by Edman degradation. Using degenerate primers designed against the amino acid sequences, a homologous probe for the chloroplast enzyme was amplified and used to isolate several full-size cDNA clones. Chloroplast triosephosphate isomerase is encoded by a single gene in spinach. Analysis of the chloroplast cDNA sequence in the context of its homologues from eukaryotes and eubacteria reveals that the gene arose through duplication of its preexisting nuclear counterpart for the cytosolic enzyme during plant evolution.

Similar articles

• Higher-plant chloroplast and cytosolic fructose-1,6-bisphosphatase isoenzymes: origins via duplication rather than prokaryote-eukaryote divergence.

  Martin W, Mustafa AZ, Henze K, Schnarrenberger C. Martin W, et al. Plant Mol Biol. 1996 Nov;32(3):485-91. doi: 10.1007/BF00019100. Plant Mol Biol. 1996. PMID: 8980497

• Purification and cloning of chloroplast 6-phosphogluconate dehydrogenase from spinach. Cyanobacterial genes for chloroplast and cytosolic isoenzymes encoded in eukaryotic chromosomes.

  Krepinsky K, Plaumann M, Martin W, Schnarrenberger C. Krepinsky K, et al. Eur J Biochem. 2001 May;268(9):2678-86. doi: 10.1046/j.1432-1327.2001.02154.x. Eur J Biochem. 2001. PMID: 11322889

• Microsequencing and cDNA cloning of the Calvin cycle/OPPP enzyme ribose-5-phosphate isomerase (EC 5.3.1.6) from spinach chloroplasts.

  Martin W, Henze K, Kellerman J, Flechner A, Schnarrenberger C. Martin W, et al. Plant Mol Biol. 1996 Feb;30(4):795-805. doi: 10.1007/BF00019012. Plant Mol Biol. 1996. PMID: 8624410

• Eubacterial origin of nuclear genes for chloroplast and cytosolic glucose-6-phosphate isomerase from spinach: sampling eubacterial gene diversity in eukaryotic chromosomes through symbiosis.

  Nowitzki U, Flechner A, Kellermann J, Hasegawa M, Schnarrenberger C, Martin W. Nowitzki U, et al. Gene. 1998 Jul 3;214(1-2):205-13. doi: 10.1016/s0378-1119(98)00229-7. Gene. 1998. PMID: 9651529

• The evolution of alpha/beta barrel enzymes.

  Farber GK, Petsko GA. Farber GK, et al. Trends Biochem Sci. 1990 Jun;15(6):228-34. doi: 10.1016/0968-0004(90)90035-a. Trends Biochem Sci. 1990. PMID: 2200166 Review.

Cited by

• Linking genotype to phenotype to identify genetic variation relating to host susceptibility in the mountain pine beetle system.

  Cullingham CI, Peery RM, Fortier CE, Mahon EL, Cooke JEK, Coltman DW. Cullingham CI, et al. Evol Appl. 2019 Feb 19;13(1):48-61. doi: 10.1111/eva.12773. eCollection 2020 Jan. Evol Appl. 2019. PMID: 31892943 Free PMC article.

• Higher-plant chloroplast and cytosolic 3-phosphoglycerate kinases: a case of endosymbiotic gene replacement.

  Brinkmann H, Martin W. Brinkmann H, et al. Plant Mol Biol. 1996 Jan;30(1):65-75. doi: 10.1007/BF00017803. Plant Mol Biol. 1996. PMID: 8616244

• Cloning of the amphibolic Calvin cycle/OPPP enzyme D-ribulose-5-phosphate 3-epimerase (EC 5.1.3.1) from spinach chloroplasts: functional and evolutionary aspects.

  Nowitzki U, Wyrich R, Westhoff P, Henze K, Schnarrenberger C, Martin W. Nowitzki U, et al. Plant Mol Biol. 1995 Dec;29(6):1279-91. doi: 10.1007/BF00020468. Plant Mol Biol. 1995. PMID: 8616224

• Higher-plant chloroplast and cytosolic fructose-1,6-bisphosphatase isoenzymes: origins via duplication rather than prokaryote-eukaryote divergence.

  Martin W, Mustafa AZ, Henze K, Schnarrenberger C. Martin W, et al. Plant Mol Biol. 1996 Nov;32(3):485-91. doi: 10.1007/BF00019100. Plant Mol Biol. 1996. PMID: 8980497

• Characterization of stress and methylglyoxal inducible triose phosphate isomerase (OscTPI) from rice.

  Sharma S, Mustafiz A, Singla-Pareek SL, Shankar Srivastava P, Sopory SK. Sharma S, et al. Plant Signal Behav. 2012 Oct 1;7(10):1337-45. doi: 10.4161/psb.21415. Epub 2012 Aug 20. Plant Signal Behav. 2012. PMID: 22902706 Free PMC article.

References

Publication types

MeSH terms

Substances