A strand-specific endonucleolytic activity with DNA site preference for cleavage in Chlamydia trachomatis - PubMed
A strand-specific endonucleolytic activity with DNA site preference for cleavage in Chlamydia trachomatis
S A Mathews et al. J Bacteriol. 1994 Aug.
Abstract
Endonucleolytic activity was detected in high-salt extracts of Chlamydia trachomatis cell lysates. This nucleolytic activity showed specificity for one strand of a PCR fragment containing the strong chlamydial promoter that regulates synthesis of two short plasmid-specified RNAs. Strand specificity was also observed with supercoiled DNA containing the same region; once again, the same strand was cleaved. The sensitive strand is the nontemplate strand for two short transcripts which initiate at the same nucleotide. Alignment of sequences on either side of the nick sites revealed a consensus which may be required for recognition of the enzymatic activity.
Similar articles
-
Sequence specific binding of chlamydial histone H1-like protein.
Kaul R, Allen M, Bradbury EM, Wenman WM. Kaul R, et al. Nucleic Acids Res. 1996 Aug 1;24(15):2981-9. doi: 10.1093/nar/24.15.2981. Nucleic Acids Res. 1996. PMID: 8760883 Free PMC article.
-
Tan M, Engel JN. Tan M, et al. J Bacteriol. 1996 Dec;178(23):6975-82. doi: 10.1128/jb.178.23.6975-6982.1996. J Bacteriol. 1996. PMID: 8955322 Free PMC article.
-
In vitro transcription in Chlamydia psittaci and Chlamydia trachomatis.
Mathews SA, Douglas A, Sriprakash KS, Hatch TP. Mathews SA, et al. Mol Microbiol. 1993 Mar;7(6):937-46. doi: 10.1111/j.1365-2958.1993.tb01185.x. Mol Microbiol. 1993. PMID: 8483421
-
Convergent and overlapping transcripts of the Chlamydia trachomatis 7.5-kb plasmid.
Fahr MJ, Sriprakash KS, Hatch TP. Fahr MJ, et al. Plasmid. 1992 Nov;28(3):247-57. doi: 10.1016/0147-619x(92)90056-g. Plasmid. 1992. PMID: 1281330
-
Claywell JE, Fisher DJ. Claywell JE, et al. J Bacteriol. 2016 Jun 13;198(13):1827-1836. doi: 10.1128/JB.00025-16. Print 2016 Jul 1. J Bacteriol. 2016. PMID: 27114464 Free PMC article.
Cited by
-
Sequence specific binding of chlamydial histone H1-like protein.
Kaul R, Allen M, Bradbury EM, Wenman WM. Kaul R, et al. Nucleic Acids Res. 1996 Aug 1;24(15):2981-9. doi: 10.1093/nar/24.15.2981. Nucleic Acids Res. 1996. PMID: 8760883 Free PMC article.
-
Tan M, Engel JN. Tan M, et al. J Bacteriol. 1996 Dec;178(23):6975-82. doi: 10.1128/jb.178.23.6975-6982.1996. J Bacteriol. 1996. PMID: 8955322 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources