The SUP35 omnipotent suppressor gene is involved in the maintenance of the non-Mendelian determinant [psi+] in the yeast Saccharomyces cerevisiae - PubMed
The SUP35 omnipotent suppressor gene is involved in the maintenance of the non-Mendelian determinant [psi+] in the yeast Saccharomyces cerevisiae
M D Ter-Avanesyan et al. Genetics. 1994 Jul.
Abstract
The SUP35 gene of yeast Saccharomyces cerevisiae encodes a 76.5-kD ribosome-associated protein (Sup35p), the C-terminal part of which exhibits a high degree of similarity to EF-1 alpha elongation factor, while its N-terminal region is unique. Mutations in or overexpression of the SUP35 gene can generate an omnipotent suppressor effect. In the present study the SUP35 wild-type gene was replaced with deletion alleles generated in vitro that encode Sup35p lacking all or a part of the unique N-terminal region. These 5'-deletion alleles lead, in a haploid strain, simultaneously to an antisuppressor effect and to loss of the non-Mendelian determinant [psi+]. The antisuppressor effect is dominant while the elimination of the [psi+] determinant is a recessive trait. A set of the plasmid-borne deletion alleles of the SUP35 gene was tested for the ability to maintain [psi+]. It was shown that the first 114 amino acids of Sup35p are sufficient to maintain the [psi+] determinant. We propose that the Sup35p serves as a trans-acting factor required for the maintenance of [psi+].
Similar articles
-
Doel SM, McCready SJ, Nierras CR, Cox BS. Doel SM, et al. Genetics. 1994 Jul;137(3):659-70. doi: 10.1093/genetics/137.3.659. Genetics. 1994. PMID: 8088511 Free PMC article.
-
Ter-Avanesyan MD, Kushnirov VV, Dagkesamanskaya AR, Didichenko SA, Chernoff YO, Inge-Vechtomov SG, Smirnov VN. Ter-Avanesyan MD, et al. Mol Microbiol. 1993 Mar;7(5):683-92. doi: 10.1111/j.1365-2958.1993.tb01159.x. Mol Microbiol. 1993. PMID: 8469113
-
Kochneva-Pervukhova NV, Poznyakovski AI, Smirnov VN, Ter-Avanesyan MD. Kochneva-Pervukhova NV, et al. Curr Genet. 1998 Aug;34(2):146-51. doi: 10.1007/s002940050379. Curr Genet. 1998. PMID: 9724418
-
[PSI] and [URE3] as yeast prions.
Wickner RB, Masison DC, Edskes HK. Wickner RB, et al. Yeast. 1995 Dec;11(16):1671-85. doi: 10.1002/yea.320111609. Yeast. 1995. PMID: 8720070 Review.
-
[URE3] and [PSI] are prions of yeast and evidence for new fungal prions.
Masison DC, Edskes HK, Maddelein ML, Taylor KL, Wickner RB. Masison DC, et al. Curr Issues Mol Biol. 2000 Apr;2(2):51-9. Curr Issues Mol Biol. 2000. PMID: 11471564 Review.
Cited by
-
A bipolar personality of yeast prion proteins.
Kurahashi H, Oishi K, Nakamura Y. Kurahashi H, et al. Prion. 2011 Oct-Dec;5(4):305-10. doi: 10.4161/pri.18307. Epub 2011 Oct 1. Prion. 2011. PMID: 22156730 Free PMC article. Review.
-
The [URE3] prion is not conserved among Saccharomyces species.
Talarek N, Maillet L, Cullin C, Aigle M. Talarek N, et al. Genetics. 2005 Sep;171(1):23-34. doi: 10.1534/genetics.105.043489. Epub 2005 Jun 14. Genetics. 2005. PMID: 15956663 Free PMC article.
-
Allen KD, Wegrzyn RD, Chernova TA, Müller S, Newnam GP, Winslett PA, Wittich KB, Wilkinson KD, Chernoff YO. Allen KD, et al. Genetics. 2005 Mar;169(3):1227-42. doi: 10.1534/genetics.104.037168. Epub 2004 Nov 15. Genetics. 2005. PMID: 15545639 Free PMC article.
-
Dependence and independence of [PSI(+)] and [PIN(+)]: a two-prion system in yeast?
Derkatch IL, Bradley ME, Masse SV, Zadorsky SP, Polozkov GV, Inge-Vechtomov SG, Liebman SW. Derkatch IL, et al. EMBO J. 2000 May 2;19(9):1942-52. doi: 10.1093/emboj/19.9.1942. EMBO J. 2000. PMID: 10790361 Free PMC article.
-
Baxa U, Keller PW, Cheng N, Wall JS, Steven AC. Baxa U, et al. Mol Microbiol. 2011 Jan;79(2):523-32. doi: 10.1111/j.1365-2958.2010.07466.x. Epub 2010 Dec 7. Mol Microbiol. 2011. PMID: 21219467 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases