Fingerprinting G-protein-coupled receptors - PubMed

Comparative Study

Fingerprinting G-protein-coupled receptors

T K Attwood et al. Protein Eng. 1994 Feb.

Abstract

Recently we reported the design of a discriminating fingerprint for rhodopsin-like G-protein-coupled receptors (GPCRs). The fingerprint encodes the seven putative membrane-spanning motifs and was potently diagnostic of all GPCRs (52 in all) in version 8.1 of the OWL composite sequence database, readily distinguishing them from all other integral membrane proteins. With a 3-fold increase in the size of OWL, the fingerprint has been updated and now finds 332 receptors that match all the motifs. The situation, however, has grown in complexity: 61 sequences make imperfect matches with the fingerprint, yielding a total of 393 'hits'. The bulk of the partial hits are olfactory receptors: these appear to fall into discrete subfamilies in which one or more of the transmembrane motifs are either poorly matched or are not matched at all. These results are supported by preliminary phylogenetic analyses, which show the olfactory and various other partial matches clustering away from the main body of true hits. The approach has provided a powerful diagnostic tool for identifying GPCRs, and results are consistent with previous observations that the pheromone, cAMP and secretin-like receptors belong to separate families--these bear their own unique sequence fingerprints by which they may be distinguished from the rhodopsin-like superfamily.

Similar articles

• Design of a discriminating fingerprint for G-protein-coupled receptors.

  Attwood TK, Findlay JB. Attwood TK, et al. Protein Eng. 1993 Feb;6(2):167-76. doi: 10.1093/protein/6.2.167. Protein Eng. 1993. PMID: 8386361

• The G-protein-coupled receptors in the human genome form five main families. Phylogenetic analysis, paralogon groups, and fingerprints.

  Fredriksson R, Lagerström MC, Lundin LG, Schiöth HB. Fredriksson R, et al. Mol Pharmacol. 2003 Jun;63(6):1256-72. doi: 10.1124/mol.63.6.1256. Mol Pharmacol. 2003. PMID: 12761335

• Cloning of a putative G-protein-coupled receptor from Arabidopsis thaliana.

  Josefsson LG, Rask L. Josefsson LG, et al. Eur J Biochem. 1997 Oct 15;249(2):415-20. doi: 10.1111/j.1432-1033.1997.t01-1-00415.x. Eur J Biochem. 1997. PMID: 9370348

• Advances in determination of a high-resolution three-dimensional structure of rhodopsin, a model of G-protein-coupled receptors (GPCRs).

  Teller DC, Okada T, Behnke CA, Palczewski K, Stenkamp RE. Teller DC, et al. Biochemistry. 2001 Jul 3;40(26):7761-72. doi: 10.1021/bi0155091. Biochemistry. 2001. PMID: 11425302 Free PMC article. Review. No abstract available.

• Rhodopsin crystal: new template yielding realistic models of G-protein-coupled receptors?

  Archer E, Maigret B, Escrieut C, Pradayrol L, Fourmy D. Archer E, et al. Trends Pharmacol Sci. 2003 Jan;24(1):36-40. doi: 10.1016/s0165-6147(02)00009-3. Trends Pharmacol Sci. 2003. PMID: 12498729 Review.

Cited by

• SARConnect: A Tool to Interrogate the Connectivity Between Proteins, Chemical Structures and Activity Data.

  Eriksson M, Nilsson I, Kogej T, Southan C, Johansson M, Tyrchan C, Muresan S, Blomberg N, Bjäreland M. Eriksson M, et al. Mol Inform. 2012 Aug;31(8):555-568. doi: 10.1002/minf.201200030. Epub 2012 Aug 7. Mol Inform. 2012. PMID: 23308082 Free PMC article.

• Born blonde: a recessive loss-of-function mutation in the melanocortin 1 receptor is associated with cream coat coloration in Antarctic fur seals.

  Peters L, Humble E, Kröcker N, Fuchs B, Forcada J, Hoffman JI. Peters L, et al. Ecol Evol. 2016 Jul 22;6(16):5705-17. doi: 10.1002/ece3.2290. eCollection 2016 Aug. Ecol Evol. 2016. PMID: 27547348 Free PMC article.

• Multi-functionality of proteins involved in GPCR and G protein signaling: making sense of structure-function continuum with intrinsic disorder-based proteoforms.

  Fonin AV, Darling AL, Kuznetsova IM, Turoverov KK, Uversky VN. Fonin AV, et al. Cell Mol Life Sci. 2019 Nov;76(22):4461-4492. doi: 10.1007/s00018-019-03276-1. Epub 2019 Aug 19. Cell Mol Life Sci. 2019. PMID: 31428838 Free PMC article. Review.

• GPR125 positively regulates osteoclastogenesis potentially through AKT-NF-κB and MAPK signaling pathways.

  Tang CY, Wang H, Zhang Y, Wang Z, Zhu G, McVicar A, Li YP, Chen W. Tang CY, et al. Int J Biol Sci. 2022 Mar 6;18(6):2392-2405. doi: 10.7150/ijbs.70620. eCollection 2022. Int J Biol Sci. 2022. PMID: 35414778 Free PMC article.

• An indel in transmembrane helix 2 helps to trace the molecular evolution of class A G-protein-coupled receptors.

  Devillé J, Rey J, Chabbert M. Devillé J, et al. J Mol Evol. 2009 May;68(5):475-89. doi: 10.1007/s00239-009-9214-9. Epub 2009 Apr 9. J Mol Evol. 2009. PMID: 19357801

Publication types

MeSH terms

Substances

LinkOut - more resources

• Other Literature Sources

  • The Lens - Patent Citations Database