A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II - PubMed
- ️Sat Jan 01 1994
A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II
Y J Kim et al. Cell. 1994.
Abstract
A mediator was isolated from yeast that enabled a response to the activator proteins GAL4-VP16 and GCN4 in a transcription system reconstituted with essentially homogeneous basal factors and RNA polymerase II. The mediator comprised some 20 polypeptides, including the three subunits of TFIIF and other polypeptides cross-reactive with antisera against GAL11, SUG1, SRB2, SRB4, SRB5, and SRB6 proteins. Mediator not only enabled activated transcription but also conferred 8-fold greater activity in basal transcription and 12-fold greater efficiency of phosphorylation of RNA polymerase II by the TFIIH-associated C-terminal repeat domain (CTD) kinase, indicative of mediator-CTD interaction. A holoenzyme form of RNA polymerase II was independently isolated that supported a response to activator proteins with purified basal factors. The holoenzyme proved to consist of mediator associated with core 12-subunit RNA polymerase II.
Similar articles
-
An activator binding module of yeast RNA polymerase II holoenzyme.
Lee YC, Park JM, Min S, Han SJ, Kim YJ. Lee YC, et al. Mol Cell Biol. 1999 Apr;19(4):2967-76. doi: 10.1128/MCB.19.4.2967. Mol Cell Biol. 1999. PMID: 10082564 Free PMC article.
-
Mutual targeting of mediator and the TFIIH kinase Kin28.
Guidi BW, Bjornsdottir G, Hopkins DC, Lacomis L, Erdjument-Bromage H, Tempst P, Myers LC. Guidi BW, et al. J Biol Chem. 2004 Jul 9;279(28):29114-20. doi: 10.1074/jbc.M404426200. Epub 2004 May 4. J Biol Chem. 2004. PMID: 15126497
-
Mediator of transcriptional regulation.
Björklund S, Kim YJ. Björklund S, et al. Trends Biochem Sci. 1996 Sep;21(9):335-7. doi: 10.1016/s0968-0004(96)10051-7. Trends Biochem Sci. 1996. PMID: 8870496 Review.
-
Yeast RNA polymerase II holoenzyme.
Li Y, Bjorklund S, Kim YJ, Kornberg RD. Li Y, et al. Methods Enzymol. 1996;273:172-5. doi: 10.1016/s0076-6879(96)73017-3. Methods Enzymol. 1996. PMID: 8791610 Review. No abstract available.
Cited by
-
Wang F, Marshall CB, Ikura M. Wang F, et al. Cell Mol Life Sci. 2013 Nov;70(21):3989-4008. doi: 10.1007/s00018-012-1254-4. Epub 2013 Jan 11. Cell Mol Life Sci. 2013. PMID: 23307074 Free PMC article. Review.
-
The Mediator complex and transcription elongation.
Conaway RC, Conaway JW. Conaway RC, et al. Biochim Biophys Acta. 2013 Jan;1829(1):69-75. doi: 10.1016/j.bbagrm.2012.08.017. Epub 2012 Sep 13. Biochim Biophys Acta. 2013. PMID: 22983086 Free PMC article. Review.
-
Context-dependent dual role of SKI8 homologs in mRNA synthesis and turnover.
Dorcey E, Rodriguez-Villalon A, Salinas P, Santuari L, Pradervand S, Harshman K, Hardtke CS. Dorcey E, et al. PLoS Genet. 2012;8(4):e1002652. doi: 10.1371/journal.pgen.1002652. Epub 2012 Apr 12. PLoS Genet. 2012. PMID: 22511887 Free PMC article.
-
Role for the MED21-MED7 Hinge in Assembly of the Mediator-RNA Polymerase II Holoenzyme.
Sato S, Tomomori-Sato C, Tsai KL, Yu X, Sardiu M, Saraf A, Washburn MP, Florens L, Asturias FJ, Conaway RC, Conaway JW. Sato S, et al. J Biol Chem. 2016 Dec 23;291(52):26886-26898. doi: 10.1074/jbc.M116.756098. Epub 2016 Nov 7. J Biol Chem. 2016. PMID: 27821593 Free PMC article.
-
Zhou C, Knipe DM. Zhou C, et al. J Virol. 2002 Jun;76(12):5893-904. doi: 10.1128/jvi.76.12.5893-5904.2002. J Virol. 2002. PMID: 12021322 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous