The DNA-binding domain of simian virus 40 tumor antigen has multiple functions - PubMed

Comparative Study

The DNA-binding domain of simian virus 40 tumor antigen has multiple functions

K Wun-Kim et al. J Virol. 1993 Dec.

Abstract

The DNA-binding domain of simian virus 40 tumor antigen has been previously shown to participate in a number of different activities. Besides being involved in binding to sequences at the viral replication origin, this domain appears to be required for nonspecific DNA binding, for structurally distorting origin DNA (melting and untwisting), and possibly for oligomerization of the protein into hexamers and double hexamers. We now provide evidence that it also takes part in unwinding origin DNA sequences, contributes a function specifically related to in vivo DNA replication, and perhaps supports the assembly of the virus or release of the virus from the cell. This 100-amino-acid domain appears to be an excellent model system for studying how a small region of a protein could have a number of distinct activities.

Similar articles

• The zinc finger region of simian virus 40 large T antigen is needed for hexamer assembly and origin melting.

  Loeber G, Stenger JE, Ray S, Parsons RE, Anderson ME, Tegtmeyer P. Loeber G, et al. J Virol. 1991 Jun;65(6):3167-74. doi: 10.1128/JVI.65.6.3167-3174.1991. J Virol. 1991. PMID: 1851875 Free PMC article.

• Mutational analysis of simian virus 40 T-antigen primosome activities in viral DNA replication.

  Ott RD, Wang Y, Fanning E. Ott RD, et al. J Virol. 2002 May;76(10):5121-30. doi: 10.1128/jvi.76.10.5121-5130.2002. J Virol. 2002. PMID: 11967327 Free PMC article.

• Conformational rearrangements of SV40 large T antigen during early replication events.

  Cuesta I, Núñez-Ramírez R, Scheres SH, Gai D, Chen XS, Fanning E, Carazo JM. Cuesta I, et al. J Mol Biol. 2010 Apr 16;397(5):1276-86. doi: 10.1016/j.jmb.2010.02.042. Epub 2010 Feb 26. J Mol Biol. 2010. PMID: 20219473 Free PMC article.

• The initiation of simian virus 40 DNA replication in vitro.

  Bullock PA. Bullock PA. Crit Rev Biochem Mol Biol. 1997;32(6):503-68. doi: 10.3109/10409239709082001. Crit Rev Biochem Mol Biol. 1997. PMID: 9444478 Review.

• Binding and unwinding--how T antigen engages the SV40 origin of DNA replication.

  Borowiec JA, Dean FB, Bullock PA, Hurwitz J. Borowiec JA, et al. Cell. 1990 Jan 26;60(2):181-4. doi: 10.1016/0092-8674(90)90730-3. Cell. 1990. PMID: 2153460 Review. No abstract available.

Cited by

• An N-terminal deletion mutant of simian virus 40 (SV40) large T antigen oligomerizes incorrectly on SV40 DNA but retains the ability to bind to DNA polymerase alpha and replicate SV40 DNA in vitro.

  Weisshart K, Bradley MK, Weiner BM, Schneider C, Moarefi I, Fanning E, Arthur AK. Weisshart K, et al. J Virol. 1996 Jun;70(6):3509-16. doi: 10.1128/JVI.70.6.3509-3516.1996. J Virol. 1996. PMID: 8648684 Free PMC article.

• Mechanisms of simian virus 40 T-antigen activation by phosphorylation of threonine 124.

  McVey D, Woelker B, Tegtmeyer P. McVey D, et al. J Virol. 1996 Jun;70(6):3887-93. doi: 10.1128/JVI.70.6.3887-3893.1996. J Virol. 1996. PMID: 8648725 Free PMC article.

• Activation of CREB/ATF sites by polyomavirus large T antigen.

  Love TM, de Jesus R, Kean JA, Sheng Q, Leger A, Schaffhausen B. Love TM, et al. J Virol. 2005 Apr;79(7):4180-90. doi: 10.1128/JVI.79.7.4180-4190.2005. J Virol. 2005. PMID: 15767419 Free PMC article.

• Identification of a short, hydrophilic amino acid sequence critical for origin recognition by the bovine papillomavirus E1 protein.

  Gonzalez A, Bazaldua-Hernandez C, West M, Woytek K, Wilson VG. Gonzalez A, et al. J Virol. 2000 Jan;74(1):245-53. doi: 10.1128/jvi.74.1.245-253.2000. J Virol. 2000. PMID: 10590112 Free PMC article.

References

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central