pubmed.ncbi.nlm.nih.gov

Polypurine sequences within a downstream exon function as a splicing enhancer - PubMed

Polypurine sequences within a downstream exon function as a splicing enhancer

K Tanaka et al. Mol Cell Biol. 1994 Feb.

Abstract

We have previously shown that a purine-rich sequence located within exon M2 of the mouse immunoglobulin mu gene functions as a splicing enhancer, as judged by its ability to stimulate splicing of a distant upstream intron. This sequence element has been designated ERS (exon recognition sequence). In this study, we investigated the stimulatory effects of various ERS-like sequences, using the in vitro splicing system with HeLa cell nuclear extracts. Here, we show that purine-rich sequences of several natural exons that have previously been shown to be required for splicing function as a splicing enhancer like the ERS of the immunoglobulin mu gene. Moreover, even synthetic polypurine sequences had stimulatory effects on the upstream splicing. Evaluation of the data obtained from the analyses of both natural and synthetic purine-rich sequences shows that (i) alternating purine sequences can stimulate splicing, while poly(A) or poly(G) sequences cannot, and (ii) the presence of U residues within the polypurine sequence greatly reduces the level of stimulation. Competition experiments strongly suggest that the stimulatory effects of various purine-rich sequences are mediated by the same trans-acting factor(s). We conclude from these results that the purine-rich sequences that we examined in this study also represent examples of ERS. Thus, ERS is considered a general splicing element that is present in various exons and plays an important role in splice site selection.

PubMed Disclaimer

Cited by

Identification of Cryptic Novel α-Galactosidase A Gene Mutations: Abnormal mRNA Splicing and Large Deletions.
Higuchi T, Kobayashi M, Ogata J, Kaneshiro E, Shimada Y, Kobayashi H, Eto Y, Maeda S, Ohtake A, Ida H, Ohashi T. Higuchi T, et al. JIMD Rep. 2016;30:63-72. doi: 10.1007/8904_2015_475. Epub 2016 Jun 3. JIMD Rep. 2016. PMID: 27255140 Free PMC article.
Adaptive Gene Loss? Tracing Back the Pseudogenization of the Rabbit CCL8 Chemokine.
van der Loo W, Magalhaes MJ, de Matos AL, Abrantes J, Yamada F, Esteves PJ. van der Loo W, et al. J Mol Evol. 2016 Aug;83(1-2):12-25. doi: 10.1007/s00239-016-9747-7. Epub 2016 Jun 15. J Mol Evol. 2016. PMID: 27306379
Improvement of HIV-1 and Human T Cell Lymphotropic Virus Type 1 Replication-Dependent Vectors via Optimization of Reporter Gene Reconstitution and Modification with Intronic Short Hairpin RNA.
Shunaeva A, Potashnikova D, Pichugin A, Mishina A, Filatov A, Nikolaitchik O, Hu WS, Mazurov D. Shunaeva A, et al. J Virol. 2015 Oct;89(20):10591-601. doi: 10.1128/JVI.01940-15. Epub 2015 Aug 12. J Virol. 2015. PMID: 26269177 Free PMC article.
Sequence elements surrounding the acceptor site suppress alternative splicing of the sarco/endoplasmic reticulum Ca2+-ATPase 2 gene transcript.
Van Den Bosch L, Mertens L, Gijsbers S, Heyen MV, Wuytack F, Eggermont J. Van Den Bosch L, et al. Biochem J. 1997 Mar 15;322 ( Pt 3)(Pt 3):885-91. doi: 10.1042/bj3220885. Biochem J. 1997. PMID: 9148765 Free PMC article.
Identification of alternative splicing and negative splicing activity of a nonsegmented negative-strand RNA virus, Borna disease virus.
Tomonaga K, Kobayashi T, Lee BJ, Watanabe M, Kamitani W, Ikuta K. Tomonaga K, et al. Proc Natl Acad Sci U S A. 2000 Nov 7;97(23):12788-93. doi: 10.1073/pnas.97.23.12788. Proc Natl Acad Sci U S A. 2000. PMID: 11070091 Free PMC article.

References

1. Gene Expr. 1991;1(3):175-84 - PubMed
1. Mol Cell Biol. 1991 Dec;11(12):6075-83 - PubMed
1. J Biochem. 1987 Nov;102(5):1289-301 - PubMed
1. J Biol Chem. 1992 Jul 25;267(21):14902-8 - PubMed
1. Genes Dev. 1988 Mar;2(3):319-29 - PubMed

Polypurine sequences within a downstream exon function as a splicing enhancer - PubMed