Identification of a ten-amino acid proline-rich SH3 binding site - PubMed

Affiliations

• PMID: 8438166
• DOI: 10.1126/science.8438166

Comparative Study

Identification of a ten-amino acid proline-rich SH3 binding site

R Ren et al. Science. 1993.

Abstract

The Src homology 3 (SH3) region is a small protein domain present in a very large group of proteins, including cytoskeletal elements and signaling proteins. It is believed that SH3 domains serve as modules that mediate protein-protein associations and, along with Src homology 2 (SH2) domains, regulate cytoplasmic signaling. The SH3 binding sites of two SH3 binding proteins were localized to a nine- or ten-amino acid stretch very rich in proline residues. Similar SH3 binding motifs exist in the formins, proteins that function in pattern formation in embryonic limbs of the mouse, and one subtype of the muscarinic acetylcholine receptor. Identification of the SH3 binding site provides a basis for understanding the interaction between the SH3 domains and their targets.

Similar articles

• Proline-rich sequences that bind to Src homology 3 domains with individual specificities.

  Alexandropoulos K, Cheng G, Baltimore D. Alexandropoulos K, et al. Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3110-4. doi: 10.1073/pnas.92.8.3110. Proc Natl Acad Sci U S A. 1995. PMID: 7536925 Free PMC article.

• Formin binding proteins bear WWP/WW domains that bind proline-rich peptides and functionally resemble SH3 domains.

  Chan DC, Bedford MT, Leder P. Chan DC, et al. EMBO J. 1996 Mar 1;15(5):1045-54. EMBO J. 1996. PMID: 8605874 Free PMC article.

• Identification of a protein that binds to the SH3 region of Abl and is similar to Bcr and GAP-rho.

  Cicchetti P, Mayer BJ, Thiel G, Baltimore D. Cicchetti P, et al. Science. 1992 Aug 7;257(5071):803-6. doi: 10.1126/science.1379745. Science. 1992. PMID: 1379745

• SH2 and SH3 domains as molecular adhesives: the interactions of Crk and Abl.

  Feller SM, Ren R, Hanafusa H, Baltimore D. Feller SM, et al. Trends Biochem Sci. 1994 Nov;19(11):453-8. doi: 10.1016/0968-0004(94)90129-5. Trends Biochem Sci. 1994. PMID: 7855886 Review.

• WW and SH3 domains, two different scaffolds to recognize proline-rich ligands.

  Macias MJ, Wiesner S, Sudol M. Macias MJ, et al. FEBS Lett. 2002 Feb 20;513(1):30-7. doi: 10.1016/s0014-5793(01)03290-2. FEBS Lett. 2002. PMID: 11911877 Review.

Cited by

• The role of SH3BP2 in the pathophysiology of cherubism.

  Reichenberger EJ, Levine MA, Olsen BR, Papadaki ME, Lietman SA. Reichenberger EJ, et al. Orphanet J Rare Dis. 2012 May 24;7 Suppl 1(Suppl 1):S5. doi: 10.1186/1750-1172-7-S1-S5. Epub 2012 May 24. Orphanet J Rare Dis. 2012. PMID: 22640988 Free PMC article. Review.

• Computational analysis and prediction of the binding motif and protein interacting partners of the Abl SH3 domain.

  Hou T, Chen K, McLaughlin WA, Lu B, Wang W. Hou T, et al. PLoS Comput Biol. 2006 Jan;2(1):e1. doi: 10.1371/journal.pcbi.0020001. Epub 2006 Jan 27. PLoS Comput Biol. 2006. PMID: 16446784 Free PMC article.

• Regulation of actin polymerization and adhesion-dependent cell edge protrusion by the Abl-related gene (Arg) tyrosine kinase and N-WASp.

  Miller MM, Lapetina S, MacGrath SM, Sfakianos MK, Pollard TD, Koleske AJ. Miller MM, et al. Biochemistry. 2010 Mar 16;49(10):2227-34. doi: 10.1021/bi901721u. Biochemistry. 2010. PMID: 20146487 Free PMC article.

• Functional analysis of a human homologue of the Drosophila actin binding protein anillin suggests a role in cytokinesis.

  Oegema K, Savoian MS, Mitchison TJ, Field CM. Oegema K, et al. J Cell Biol. 2000 Aug 7;150(3):539-52. doi: 10.1083/jcb.150.3.539. J Cell Biol. 2000. PMID: 10931866 Free PMC article.

• Human sphingosine kinase: purification, molecular cloning and characterization of the native and recombinant enzymes.

  Pitson SM, D'andrea RJ, Vandeleur L, Moretti PA, Xia P, Gamble JR, Vadas MA, Wattenberg BW. Pitson SM, et al. Biochem J. 2000 Sep 1;350 Pt 2(Pt 2):429-41. Biochem J. 2000. PMID: 10947957 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Ovid Technologies, Inc.

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc
  • Gene Ontology
  • GlyGen glycoinformatics resource
  • Mouse Genome Informatics (MGI)

• Miscellaneous

  • NCI CPTAC Assay Portal