Diffusion across the nuclear envelope inhibited by depletion of the nuclear Ca2+ store - PubMed

Diffusion across the nuclear envelope inhibited by depletion of the nuclear Ca2+ store

L Stehno-Bittel et al. Science. 1995.

Abstract

Intact, isolated nuclei and a nuclear membrane (ghost) preparation were used to study regulation of the movement of small molecules across the Xenopus laevis oocyte nuclear membrane. In contrast to models of the nuclear pore complex, which assume passive bidirectional diffusion of molecules less than 70 kilodaltons, diffusion of intermediate-sized molecules was regulated by the nuclear envelope calcium stores. After depletion of nuclear store calcium by inositol 1,4,5-trisphosphate or calcium chelators, fluorescent molecules conjugated to 10-kilodalton dextran were unable to enter the nucleus. Dye exclusion after calcium store depletion was not dependent on the nuclear matrix because it occurred in nuclear ghosts lacking nucleoplasm. Smaller molecules and ions (500-dalton Lucifer yellow and manganese) diffused freely into the core of the nuclear ghosts and intact nuclei even after calcium store depletion. Thus, depletion of the nuclear calcium store blocks diffusion of intermediate-sized molecules.

Similar articles

• Nuclear calcium and the regulation of the nuclear pore complex.

  Perez-Terzic C, Jaconi M, Clapham DE. Perez-Terzic C, et al. Bioessays. 1997 Sep;19(9):787-92. doi: 10.1002/bies.950190908. Bioessays. 1997. PMID: 9297969 Review.

• Conformational states of the nuclear pore complex induced by depletion of nuclear Ca2+ stores.

  Perez-Terzic C, Pyle J, Jaconi M, Stehno-Bittel L, Clapham DE. Perez-Terzic C, et al. Science. 1996 Sep 27;273(5283):1875-7. doi: 10.1126/science.273.5283.1875. Science. 1996. PMID: 8791595

• Passive transport of macromolecules through Xenopus laevis nuclear envelope.

  Enss K, Danker T, Schlune A, Buchholz I, Oberleithner H. Enss K, et al. J Membr Biol. 2003 Dec 1;196(3):147-55. doi: 10.1007/s00232-003-0632-0. J Membr Biol. 2003. PMID: 14724740

• Activation of ryanodine receptors in the nuclear envelope alters the conformation of the nuclear pore complex.

  Erickson ES, Mooren OL, Moore-Nichols D, Dunn RC. Erickson ES, et al. Biophys Chem. 2004 Dec 1;112(1):1-7. doi: 10.1016/j.bpc.2004.06.010. Biophys Chem. 2004. PMID: 15501570

• Calcium regulation of nuclear pore permeability.

  Lee MA, Dunn RC, Clapham DE, Stehno-Bittel L. Lee MA, et al. Cell Calcium. 1998 Feb-Mar;23(2-3):91-101. doi: 10.1016/s0143-4160(98)90107-5. Cell Calcium. 1998. PMID: 9601604 Review.

Cited by

• Calcium signaling in synapse-to-nucleus communication.

  Hagenston AM, Bading H. Hagenston AM, et al. Cold Spring Harb Perspect Biol. 2011 Nov 1;3(11):a004564. doi: 10.1101/cshperspect.a004564. Cold Spring Harb Perspect Biol. 2011. PMID: 21791697 Free PMC article. Review.

• Conformational changes of the in situ nuclear pore complex.

  Wang H, Clapham DE. Wang H, et al. Biophys J. 1999 Jul;77(1):241-7. doi: 10.1016/S0006-3495(99)76885-2. Biophys J. 1999. PMID: 10388753 Free PMC article.

• Kinetics of protein import into isolated Xenopus oocyte nuclei.

  Radtke T, Schmalz D, Coutavas E, Soliman TM, Peters R. Radtke T, et al. Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2407-12. doi: 10.1073/pnas.051616598. Epub 2001 Feb 20. Proc Natl Acad Sci U S A. 2001. PMID: 11226252 Free PMC article.

• Dendrimer-assisted patch-clamp sizing of nuclear pores.

  Bustamante JO, Michelette ER, Geibel JP, Hanover JA, McDonnell TJ, Dean DA. Bustamante JO, et al. Pflugers Arch. 2000 Apr;439(6):829-37. doi: 10.1007/s004249900233. Pflugers Arch. 2000. PMID: 10784359 Free PMC article.

• Calcium regulation of nucleocytoplasmic transport.

  Sarma A, Yang W. Sarma A, et al. Protein Cell. 2011 Apr;2(4):291-302. doi: 10.1007/s13238-011-1038-x. Epub 2011 Apr 27. Protein Cell. 2011. PMID: 21528351 Free PMC article. Review.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Ovid Technologies, Inc.

• Miscellaneous

  • NCI CPTAC Assay Portal