Division of Listeria monocytogenes serovar 4b strains into two groups by PCR and restriction enzyme analysis - PubMed

Division of Listeria monocytogenes serovar 4b strains into two groups by PCR and restriction enzyme analysis

H Ericsson et al. Appl Environ Microbiol. 1995 Nov.

Abstract

Altogether, 133 strains of Listeria monocytogenes serovar 4b were investigated. A segment of 2,916 bp containing parts of the two genes inlA and inlB in L. monocytogenes was amplified by the PCR technique. The PCR product obtained was cleaved with the restriction enzyme AluI, and the fragments generated were separated by gel electrophoresis, leading to two distinct groups: PCR-restriction enzyme analysis groups I and II, containing 37 and 96 strains, respectively. The PCR-restriction enzyme analysis method described in this paper could be a useful tool for the subtyping of L. monocytogenes serovar 4b strains.

Similar articles

• Division of Listeria monocytogenes serovar 1/2a strains into two groups by PCR and restriction enzyme analysis.

  Unnerstad H, Nilsson I, Ericsson H, Danielsson-Tham ML, Bille J, Bannerman E, Tham W. Unnerstad H, et al. Appl Environ Microbiol. 1999 May;65(5):2054-6. doi: 10.1128/AEM.65.5.2054-2056.1999. Appl Environ Microbiol. 1999. PMID: 10224000 Free PMC article.

• A RE-PCR method to distinguish Listeria monocytogenes serovars.

  Comi G, Cocolin L, Cantoni C, Manzano M. Comi G, et al. FEMS Immunol Med Microbiol. 1997 Jun;18(2):99-104. doi: 10.1111/j.1574-695X.1997.tb01033.x. FEMS Immunol Med Microbiol. 1997. PMID: 9223613

• Comparison of ribotyping, arbitrarily primed PCR, and pulsed-field gel electrophoresis for molecular typing of Listeria monocytogenes.

  Louie M, Jayaratne P, Luchsinger I, Devenish J, Yao J, Schlech W, Simor A. Louie M, et al. J Clin Microbiol. 1996 Jan;34(1):15-9. doi: 10.1128/jcm.34.1.15-19.1996. J Clin Microbiol. 1996. PMID: 8748263 Free PMC article.

• DNA polymorphism in strains of Listeria monocytogenes.

  Carriere C, Allardet-Servent A, Bourg G, Audurier A, Ramuz M. Carriere C, et al. J Clin Microbiol. 1991 Jul;29(7):1351-5. doi: 10.1128/jcm.29.7.1351-1355.1991. J Clin Microbiol. 1991. PMID: 1715882 Free PMC article.

• Typing of food-borne Listeria monocytogenes by polymerase chain reaction-restriction enzyme analysis and amplified fragment length polymorphism.

  Mikasová E, Oravcová K, Kaclíková E, Kuchta T, Drahovská H. Mikasová E, et al. New Microbiol. 2005 Jul;28(3):265-70. New Microbiol. 2005. PMID: 16240700

Cited by

• Pyrosequencing as a method for grouping of Listeria monocytogenes strains on the basis of single-nucleotide polymorphisms in the inlB gene.

  Unnerstad H, Ericsson H, Alderborn A, Tham W, Danielsson-Tham ML, Mattsson JG. Unnerstad H, et al. Appl Environ Microbiol. 2001 Nov;67(11):5339-42. doi: 10.1128/AEM.67.11.5339-5342.2001. Appl Environ Microbiol. 2001. PMID: 11679367 Free PMC article.

• Characterization of Listeria monocytogenes isolates by esterase electrophoresis.

  Harvey J, Gilmour A. Harvey J, et al. Appl Environ Microbiol. 1996 Apr;62(4):1461-6. doi: 10.1128/aem.62.4.1461-1466.1996. Appl Environ Microbiol. 1996. PMID: 8919813 Free PMC article.

• DNA fragments from regions involved in surface antigen expression specifically identify Listeria monocytogenes serovar 4 and a subset thereof: cluster IIB (serotypes 4b, 4d, and 4e).

  Lei XH, Promadej N, Kathariou S. Lei XH, et al. Appl Environ Microbiol. 1997 Mar;63(3):1077-82. doi: 10.1128/aem.63.3.1077-1082.1997. Appl Environ Microbiol. 1997. PMID: 9055423 Free PMC article.

• Use of PCR-restriction fragment length polymorphism of inlA for rapid screening of Listeria monocytogenes strains deficient in the ability to invade Caco-2 cells.

  Rousseaux S, Olier M, Lemaître JP, Piveteau P, Guzzo J. Rousseaux S, et al. Appl Environ Microbiol. 2004 Apr;70(4):2180-5. doi: 10.1128/AEM.70.4.2180-2185.2004. Appl Environ Microbiol. 2004. PMID: 15066811 Free PMC article.

References

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central