Expression of gonadotropin-releasing hormone and gonadotropin-releasing hormone receptor mRNAs in various non-reproductive human tissues - PubMed

Affiliations

• PMID: 8529206

Expression of gonadotropin-releasing hormone and gonadotropin-releasing hormone receptor mRNAs in various non-reproductive human tissues

S S Kakar et al. Cancer Lett. 1995.

Abstract

Recently, cloning of the gonadotropin-releasing hormone (GnRH) receptor from the human breast tumor cell line (MCF-7) and from an ovarian tumor, and its expression in various other human tumors, tumor cell lines and reproductive organs have been reported (Kakar et al., Mol. Cell. Endocrinol., 106 (1994) 145-149). In the present studies, we investigated the expression of GnRH and GnRH receptor mRNAs in normal human non-reproductive tissues. Using reverse transcriptase-polymerase chain reaction (RT-PCR) techniques and specific oligonucleotide primers derived from the placental GnRH cDNA sequence, PCR products of the expected size were obtained from human liver, heart, skeletal muscle, kidney, placenta, and pituitary. The authenticity of the PCR products was confirmed by Southern blot analysis with an internal oligonucleotide primer as probe. Similarly, using specific oligonucleotide primers for the GnRH receptor selected from the human pituitary GnRH receptor cDNA sequence, PCR products of the expected size were amplified from human liver, heart, skeletal muscle, kidney, placenta, and pituitary, and these strongly hybridized with the human GnRH receptor cDNA on Southern blot. Cloning and nucleotide sequencing of the PCR products for the GnRH and GnRH receptor from heart revealed identical sequences when compared to the human placental GnRH and pituitary GnRH receptor cDNAs, respectively. These data demonstrate for the first time the existence of GnRH and GnRH receptor mRNAs in normal human non-reproductive tissues and suggest that GnRH and its receptor may play an important role in the regulation of cellular functions in an autocrine or paracrine manner, in addition to regulating the secretion of gonadotropins from the anterior pituitary.

Similar articles

• mRNA expression of gonadotropin-releasing hormones (GnRHs) and GnRH receptor in goldfish.

  Yu KL, He ML, Chik CC, Lin XW, Chang JP, Peter RE. Yu KL, et al. Gen Comp Endocrinol. 1998 Dec;112(3):303-11. doi: 10.1006/gcen.1998.7137. Gen Comp Endocrinol. 1998. PMID: 9843636

• Expression and regulation of gonadotropin-releasing hormone (GnRH) and GnRH receptor messenger ribonucleic acids in human granulosa-luteal cells.

  Peng C, Fan NC, Ligier M, Väänänen J, Leung PC. Peng C, et al. Endocrinology. 1994 Nov;135(5):1740-6. doi: 10.1210/endo.135.5.7956897. Endocrinology. 1994. PMID: 7956897

• The expression of gonadotropin-releasing hormone and its receptor in endometrial cancer, and its relevance as an autocrine growth factor.

  Chatzaki E, Bax CM, Eidne KA, Anderson L, Grudzinskas JG, Gallagher CJ. Chatzaki E, et al. Cancer Res. 1996 May 1;56(9):2059-65. Cancer Res. 1996. PMID: 8616851

• [GnRH receptor and the regulation of its gene expression].

  Han XB, Cao YQ, Zhuang LZ. Han XB, et al. Sheng Li Ke Xue Jin Zhan. 1998 Jul;29(3):198-202. Sheng Li Ke Xue Jin Zhan. 1998. PMID: 12501635 Review. Chinese.

• GnRH in non-hypothalamic reproductive tissues.

  Ramakrishnappa N, Rajamahendran R, Lin YM, Leung PC. Ramakrishnappa N, et al. Anim Reprod Sci. 2005 Aug;88(1-2):95-113. doi: 10.1016/j.anireprosci.2005.05.009. Anim Reprod Sci. 2005. PMID: 15982835 Review.

Cited by

• GnRH receptor expression in human prostate cancer cells is affected by hormones and growth factors.

  Angelucci C, Lama G, Iacopino F, Ferracuti S, Bono AV, Millar RP, Sica G. Angelucci C, et al. Endocrine. 2009 Aug;36(1):87-97. doi: 10.1007/s12020-009-9195-x. Epub 2009 Apr 28. Endocrine. 2009. PMID: 19399647

• Conventional and new proposals of GnRH therapy for ovarian, breast, and prostatic cancers.

  Garrido MP, Hernandez A, Vega M, Araya E, Romero C. Garrido MP, et al. Front Endocrinol (Lausanne). 2023 Mar 28;14:1143261. doi: 10.3389/fendo.2023.1143261. eCollection 2023. Front Endocrinol (Lausanne). 2023. PMID: 37056674 Free PMC article. Review.

• Gonadotropin-Releasing Hormone (GnRH) and Its Agonists in Bovine Reproduction I: Structure, Biosynthesis, Physiological Effects, and Its Role in Estrous Synchronization.

  Hassanein EM, Szelényi Z, Szenci O. Hassanein EM, et al. Animals (Basel). 2024 May 15;14(10):1473. doi: 10.3390/ani14101473. Animals (Basel). 2024. PMID: 38791690 Free PMC article. Review.

• Mutual Interactions Between GnRH and Kisspeptin in GnRH- and Kiss-1-Expressing Immortalized Hypothalamic Cell Models.

  Kanasaki H, Tumurbaatar T, Tumurgan Z, Oride A, Okada H, Kyo S. Kanasaki H, et al. Reprod Sci. 2021 Dec;28(12):3380-3389. doi: 10.1007/s43032-021-00695-z. Epub 2021 Jul 15. Reprod Sci. 2021. PMID: 34268716

• Gonadotropin-Releasing Hormone Receptor (GnRHR) and Hypogonadotropic Hypogonadism.

  Fanis P, Neocleous V, Papapetrou I, Phylactou LA, Skordis N. Fanis P, et al. Int J Mol Sci. 2023 Nov 4;24(21):15965. doi: 10.3390/ijms242115965. Int J Mol Sci. 2023. PMID: 37958948 Free PMC article. Review.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • Guide to Pharmacology