A powerful new technique for isolating genes encoding cell surface antigens using retroviral expression cloning - PubMed
- ️Mon Jan 01 1996
. 1996 Jan 15;156(2):611-20.
Affiliations
- PMID: 8543812
A powerful new technique for isolating genes encoding cell surface antigens using retroviral expression cloning
A C Zannettino et al. J Immunol. 1996.
Abstract
cDNA expression cloning using retroviral vectors provides a means of stably introducing genes into target cells at efficiencies that surpass those achieved by transfection. Furthermore, retroviral vectors allow for the introduction and expression of complex cDNA libraries in a wide range of cell types, including cells of hemopoietic origin. Here we report a novel method for rapidly isolating genes encoding cell surface molecules (CSM) from a human bone marrow stromal cell cDNA library constructed in the retroviral vector, pRUFneo. With a newly described, highly efficient selection strategy using mAb and Ab-coated magnetic beads, we have successfully isolated six cDNA encoding previously defined CSM, including beta 1 integrin and endoglin. Moreover, we have used this approach to define the gene and hence the CSM identified by three previously unclustered mAb. These results confirm previous studies demonstrating the general utility of retroviral cDNA libraries and further extend their use to the expression cloning of cDNA encoding CSM.
Similar articles
-
Applications of retrovirus-mediated expression cloning.
Onishi M, Kinoshita S, Morikawa Y, Shibuya A, Phillips J, Lanier LL, Gorman DM, Nolan GP, Miyajima A, Kitamura T. Onishi M, et al. Exp Hematol. 1996 Feb;24(2):324-9. Exp Hematol. 1996. PMID: 8641361
-
Miyoshi S, Motohashi T, Nakamura Y, Osawa M, Hiroyama T, Kim DK, Tokumoto Y, Nakauchi H. Miyoshi S, et al. Biochem Biophys Res Commun. 2001 Dec 21;289(5):1192-8. doi: 10.1006/bbrc.2001.6097. Biochem Biophys Res Commun. 2001. PMID: 11741319
-
Rapid expression cloning of receptors using epitope-tagged ligands and high-speed cell sorting.
Robeva AS, Yan-Neale Y, Burfeind P, Bodian DL, Chirn GW, Kolbinger F, Labow M, Vallon RD. Robeva AS, et al. Cytometry A. 2003 Feb;51(2):59-67. doi: 10.1002/cyto.a.10012. Cytometry A. 2003. PMID: 12541280
-
Direct cloning of lambda gt11 cDNA inserts into a plasmid vector.
Poulin ML, Chiu IM. Poulin ML, et al. Methods Mol Biol. 1994;31:9-17. doi: 10.1385/0-89603-258-2:9. Methods Mol Biol. 1994. PMID: 7921042 Review. No abstract available.
-
[Insertion of genetic material into the hematopoietic cells of mammals].
Khrushchov NG. Khrushchov NG. Ontogenez. 1985 Nov-Dec;16(6):547-52. Ontogenez. 1985. PMID: 2868439 Review. Russian. No abstract available.
Cited by
-
Apo2L/TRAIL inhibits tumor growth and bone destruction in a murine model of multiple myeloma.
Labrinidis A, Diamond P, Martin S, Hay S, Liapis V, Zinonos I, Sims NA, Atkins GJ, Vincent C, Ponomarev V, Findlay DM, Zannettino AC, Evdokiou A. Labrinidis A, et al. Clin Cancer Res. 2009 Mar 15;15(6):1998-2009. doi: 10.1158/1078-0432.CCR-08-2444. Epub 2009 Mar 10. Clin Cancer Res. 2009. PMID: 19276263 Free PMC article.
-
Zinonos I, Labrinidis A, Lee M, Liapis V, Hay S, Ponomarev V, Diamond P, Zannettino AC, Findlay DM, Evdokiou A. Zinonos I, et al. Mol Cancer Ther. 2009 Oct;8(10):2969-80. doi: 10.1158/1535-7163.MCT-09-0745. Epub 2009 Oct 6. Mol Cancer Ther. 2009. PMID: 19808976 Free PMC article.
-
Zinonos I, Labrinidis A, Lee M, Liapis V, Hay S, Ponomarev V, Diamond P, Findlay DM, Zannettino AC, Evdokiou A. Zinonos I, et al. J Bone Miner Res. 2011 Mar;26(3):630-43. doi: 10.1002/jbmr.244. J Bone Miner Res. 2011. PMID: 20818644 Free PMC article.
-
Zannettino AC, Roubelakis M, Welldon KJ, Jackson DE, Simmons PJ, Bendall LJ, Henniker A, Harrison KL, Niutta S, Bradstock KF, Watt SM. Zannettino AC, et al. Biochem J. 2003 Mar 1;370(Pt 2):537-49. doi: 10.1042/BJ20020935. Biochem J. 2003. PMID: 12410637 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Medical