Inhibition of HIV-1 replication in lymphocytes by mutants of the Rev cofactor eIF-5A - PubMed

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• PMID: 8596953
• DOI: 10.1126/science.271.5257.1858

Inhibition of HIV-1 replication in lymphocytes by mutants of the Rev cofactor eIF-5A

D Bevec et al. Science. 1996.

Abstract

Eukaryotic initiation factor 5A(eIF-5A) is a cellular cofactor require d for the function of the human immunodeficiency virus type-1 (HIV-1) Rev trans-activator protein. The majority of a set of eIF-5A mutants did not support growth of yeast cells having an inactivated genomic copy of eIF-5A, indicating that the introduced mutation eliminated eIF-5A activity. Two nonfunctional mutants, eIF-5AM13 and eIF-5AM14, retained their binding capacity for the HIV-1 Rev response element:Rev complex. Both mutants were constitutively expressed in human T cells. When these T cells were infected with replication-competent HIV-1, virus replication was inhibited. The eIF-5AM13 and eIF5AM14 proteins blocked Rev trans-activation and Rev-mediated nuclear export.

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