Chronic ethanol-mediated up-regulation of the N-methyl-D-aspartate receptor polypeptide subunits in mouse cortical neurons in culture - PubMed

Affiliations

• PMID: 8663153
• DOI: 10.1074/jbc.271.23.13297

Free article

Chronic ethanol-mediated up-regulation of the N-methyl-D-aspartate receptor polypeptide subunits in mouse cortical neurons in culture

P Follesa et al. J Biol Chem. 1996.

Free article

Abstract

The goal of this study was to determine whether chronic ethanol-mediated up-regulation of the N-methyl-D-aspartate receptors (NMDAR) was associated with an augmentation of the NMDAR polypeptide subunits in the mammalian cortical neurons. The results show that chronic ethanol treatment produced an increase in the R1 and R2B polypeptide subunits. The R2A subunit was not expressed in these neurons. Chronic NMDAR antagonist ((+)-3-2-(carboxypiperazin-4-yl)propyl-1-phosphonic acid (CPP)) treatment also increased the R1 and R2B polypeptide subunits. A similar increase was observed when ethanol and CPP were used in combination. Binding studies using [3H]MK-801 ((+)-5-methyl-10, 11-dihydro-5H-dibenzo[a,d]cyclophenptan-5,10-imine maleate), a noncompetitive NMDAR antagonist, confirmed that concomitant exposure of ethanol and CPP up-regulated the NMDAR. Our results demonstrate for the first time that chronic ethanol treatment increased the NMDA receptor polypeptide subunit synthesis and that it was associated with an increase in [3H]MK-801 binding sites.

Similar articles

• Differences in NMDA receptor antagonist-induced locomotor activity and [3H]MK-801 binding sites in short-sleep and long-sleep mice.

  Velardo MJ, Simpson VJ, Zahniser NR. Velardo MJ, et al. Alcohol Clin Exp Res. 1998 Oct;22(7):1509-15. Alcohol Clin Exp Res. 1998. PMID: 9802536

• Effect of ethanol on phosphorylation of the NMDAR2B subunit in mouse cortical neurons.

  Kalluri HS, Ticku MK. Kalluri HS, et al. Brain Res Mol Brain Res. 1999 May 7;68(1-2):159-68. doi: 10.1016/s0169-328x(99)00057-1. Brain Res Mol Brain Res. 1999. PMID: 10320793

• Chronic ethanol treatment produces a selective upregulation of the NMDA receptor subunit gene expression in mammalian cultured cortical neurons.

  Hu XJ, Follesa P, Ticku MK. Hu XJ, et al. Brain Res Mol Brain Res. 1996 Mar;36(2):211-8. doi: 10.1016/0169-328x(95)00223-f. Brain Res Mol Brain Res. 1996. PMID: 8965641

• The NR2B subtype of NMDA receptor: a potential target for the treatment of alcohol dependence.

  Nagy J. Nagy J. Curr Drug Targets CNS Neurol Disord. 2004 Jun;3(3):169-79. doi: 10.2174/1568007043337409. Curr Drug Targets CNS Neurol Disord. 2004. PMID: 15180478 Review.

• N-Methyl-D-Aspartate Receptor Signaling and Function in Cardiovascular Tissues.

  McGee MA, Abdel-Rahman AA. McGee MA, et al. J Cardiovasc Pharmacol. 2016 Aug;68(2):97-105. doi: 10.1097/FJC.0000000000000398. J Cardiovasc Pharmacol. 2016. PMID: 27046337 Free PMC article. Review.

Cited by

• The molecular basis of tolerance.

  Pietrzykowski AZ, Treistman SN. Pietrzykowski AZ, et al. Alcohol Res Health. 2008;31(4):298-309. Alcohol Res Health. 2008. PMID: 23584007 Free PMC article. Review.

• The synaptic localization of NR2B-containing NMDA receptors is controlled by interactions with PDZ proteins and AP-2.

  Prybylowski K, Chang K, Sans N, Kan L, Vicini S, Wenthold RJ. Prybylowski K, et al. Neuron. 2005 Sep 15;47(6):845-57. doi: 10.1016/j.neuron.2005.08.016. Neuron. 2005. PMID: 16157279 Free PMC article.

• Sex- and subtype-specific adaptations in excitatory signaling onto deep-layer prelimbic cortical pyramidal neurons after chronic alcohol exposure.

  Hughes BA, O'Buckley TK, Boero G, Herman MA, Morrow AL. Hughes BA, et al. Neuropsychopharmacology. 2021 Oct;46(11):1927-1936. doi: 10.1038/s41386-021-01034-1. Epub 2021 May 25. Neuropsychopharmacology. 2021. PMID: 34035471 Free PMC article.

• Chronic alcohol drinking alters neuronal dendritic spines in the brain reward center nucleus accumbens.

  Zhou FC, Anthony B, Dunn KW, Lindquist WB, Xu ZC, Deng P. Zhou FC, et al. Brain Res. 2007 Feb 23;1134(1):148-61. doi: 10.1016/j.brainres.2006.11.046. Epub 2007 Jan 2. Brain Res. 2007. PMID: 17198693 Free PMC article.

• Ethanol-withdrawal seizures are controlled by tissue plasminogen activator via modulation of NR2B-containing NMDA receptors.

  Pawlak R, Melchor JP, Matys T, Skrzypiec AE, Strickland S. Pawlak R, et al. Proc Natl Acad Sci U S A. 2005 Jan 11;102(2):443-8. doi: 10.1073/pnas.0406454102. Epub 2005 Jan 3. Proc Natl Acad Sci U S A. 2005. PMID: 15630096 Free PMC article.

Publication types

MeSH terms

Substances