Molecular cloning and structural analysis of the functional mouse genomic XPG gene - PubMed

Molecular cloning and structural analysis of the functional mouse genomic XPG gene

D L Ludwig et al. Mamm Genome. 1996 Sep.

Abstract

The mouse XPG gene is a homolog of the human DNA excision repair gene known to be defective in the hereditary sun-sensitive disorder xeroderma pigmentosum (group-G). Defects in mouse XPG have been shown to directly affect the sensitivity of cultured cells to chemotherapy agents and may play a role in tumor cell drug resistance in vivo. A full-length cosmid clone of mouse XPG was isolated by complementation of the UV sensitivity and repair defect in CHO-UV135 cells. Exon mapping determined that the gene consisted of 15 exons within 32 kb of genomic DNA. Sequencing of intron-exon boundaries revealed that mouse XPG possesses a rare class of intron previously identified in only four other eukaryotic genes; it utilizes AT and AC dinucleotides instead of the expected GT and AG within the splice junctions. Promoter analysis determined that mouse XPG is expressed constitutively and probably initiates transcription from multiple start sites, yet, unlike the yeast homolog RAD2, we found no evidence that it is UVC inducible in cultured cells. Amino acid comparison with human XPG identified a highly conserved acidic region of homology not previously described.

Similar articles

• ERCC4 (XPF) encodes a human nucleotide excision repair protein with eukaryotic recombination homologs.

  Brookman KW, Lamerdin JE, Thelen MP, Hwang M, Reardon JT, Sancar A, Zhou ZQ, Walter CA, Parris CN, Thompson LH. Brookman KW, et al. Mol Cell Biol. 1996 Nov;16(11):6553-62. doi: 10.1128/MCB.16.11.6553. Mol Cell Biol. 1996. PMID: 8887684 Free PMC article.

• Human ERCC5 cDNA-cosmid complementation for excision repair and bipartite amino acid domains conserved with RAD proteins of Saccharomyces cerevisiae and Schizosaccharomyces pombe.

  MacInnes MA, Dickson JA, Hernandez RR, Learmonth D, Lin GY, Mudgett JS, Park MS, Schauer S, Reynolds RJ, Strniste GF, et al. MacInnes MA, et al. Mol Cell Biol. 1993 Oct;13(10):6393-402. doi: 10.1128/mcb.13.10.6393-6402.1993. Mol Cell Biol. 1993. PMID: 8413238 Free PMC article.

• Mutations that disable the DNA repair gene XPG in a xeroderma pigmentosum group G patient.

  Nouspikel T, Clarkson SG. Nouspikel T, et al. Hum Mol Genet. 1994 Jun;3(6):963-7. doi: 10.1093/hmg/3.6.963. Hum Mol Genet. 1994. PMID: 7951246

• Xeroderma pigmentosum and molecular cloning of DNA repair genes.

  Boulikas T. Boulikas T. Anticancer Res. 1996 Mar-Apr;16(2):693-708. Anticancer Res. 1996. PMID: 8687116 Review.

• A genetic mouse model carrying the nonfunctional xeroderma pigmentosum group G gene.

  Sun XZ, Harada YN, Zhang R, Cui C, Takahashi S, Fukui Y. Sun XZ, et al. Congenit Anom (Kyoto). 2003 Jun;43(2):133-9. doi: 10.1111/j.1741-4520.2003.tb01037.x. Congenit Anom (Kyoto). 2003. PMID: 12893972 Review.

Cited by

• The human XPG gene: gene architecture, alternative splicing and single nucleotide polymorphisms.

  Emmert S, Schneider TD, Khan SG, Kraemer KH. Emmert S, et al. Nucleic Acids Res. 2001 Apr 1;29(7):1443-52. doi: 10.1093/nar/29.7.1443. Nucleic Acids Res. 2001. PMID: 11266544 Free PMC article.

• Deficiency in the nuclease activity of xeroderma pigmentosum G in mice leads to hypersensitivity to UV irradiation.

  Tian M, Jones DA, Smith M, Shinkura R, Alt FW. Tian M, et al. Mol Cell Biol. 2004 Mar;24(6):2237-42. doi: 10.1128/MCB.24.6.2237-2242.2004. Mol Cell Biol. 2004. PMID: 14993263 Free PMC article.

• Postnatal growth failure, short life span, and early onset of cellular senescence and subsequent immortalization in mice lacking the xeroderma pigmentosum group G gene.

  Harada YN, Shiomi N, Koike M, Ikawa M, Okabe M, Hirota S, Kitamura Y, Kitagawa M, Matsunaga T, Nikaido O, Shiomi T. Harada YN, et al. Mol Cell Biol. 1999 Mar;19(3):2366-72. doi: 10.1128/MCB.19.3.2366. Mol Cell Biol. 1999. PMID: 10022922 Free PMC article.

• AT-AC pre-mRNA splicing mechanisms and conservation of minor introns in voltage-gated ion channel genes.

  Wu Q, Krainer AR. Wu Q, et al. Mol Cell Biol. 1999 May;19(5):3225-36. doi: 10.1128/MCB.19.5.3225. Mol Cell Biol. 1999. PMID: 10207048 Free PMC article. Review. No abstract available.

References

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Springer

• Molecular Biology Databases

  • BioCyc
  • Gene Ontology
  • GlyGen glycoinformatics resource
  • Mouse Genome Informatics (MGI)

• Miscellaneous

  • NCI CPTAC Assay Portal